耻垢分枝杆菌MSMEG_0918基因对……的生长并非必需。 （注：原文句子不完整，翻译时根据语境补充了“耻垢分枝杆菌MSMEG_0918基因”及“……”，以使译文更通顺）
MSMEG_0918 is not Essential for the Growth of .
作者信息
Bedru Eldana, Bhagwat Amala, Parish Tanya
机构信息
School of Medicine, University of Washington, Seattle, Washington, USA.
Center for Global Infectious Disease Research, Seattle Children's Research Institute, Seattle, Washington, USA.
出版信息
MicroPubl Biol. 2024 Feb 6;2024. doi: 10.17912/micropub.biology.000891. eCollection 2024.
Copper homeostasis plays a crucial role in mycobacteria. In , Rv0474 is a copper-responsive regulator with a copper-binding motif but its homolog in , MSMEG_0918, lacks the copper-binding motif. We generated MSMEG_0918 knockdown strains of using CRISPRi. We confirmed the strains had varying levels of expression using RT-PCR. We demonstrated that MSMEG_0918 under-expression did not alter the growth of in standard aerobic culture as compared to the wild-type. Our knockdown strains (CHROME1 and CHROME2) could be further used towards understanding the role of MSMEG_0918 in .
铜稳态在分枝杆菌中起着至关重要的作用。在[具体物种1]中，Rv0474是一种具有铜结合基序的铜响应调节因子，但其在[具体物种2]中的同源物MSMEG_0918缺乏铜结合基序。我们使用CRISPRi技术构建了[具体物种2]的MSMEG_0918基因敲低菌株。我们通过逆转录聚合酶链反应（RT-PCR）证实这些菌株具有不同水平的[具体基因]表达。我们证明，与野生型相比，MSMEG_0918表达不足不会改变[具体物种2]在标准需氧培养条件下的生长。我们构建的基因敲低菌株（CHROME1和CHROME2）可进一步用于了解MSMEG_0918在[具体物种2]中的作用。
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