Yu Guocan, Shen Yanqin, Yao Liwei, Xu Xudong
Zhejiang Tuberculosis Diagnosis and Treatment Center, Hangzhou Red Cross Hospital, Affiliated Hangzhou Chest Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, People's Republic of China.
Department of Nursing, Hangzhou Red Cross Hospital, Affiliated Hangzhou Chest Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, People's Republic of China.
Infect Drug Resist. 2024 Feb 19;17:673-682. doi: 10.2147/IDR.S442229. eCollection 2024.
This study aimed to evaluate the efficacy of nanopore sequencing for diagnosing pulmonary tuberculosis (PTB) using smear-negative clinical specimens.
We conducted a retrospective study based on a review of patient medical records to assess the accuracy of nanopore sequencing as a diagnostic tool for smear-negative PTB. Compared with clinical diagnosis, we determined the sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV), and area under the curve (AUC) of nanopore sequencing.
A total of 647 patients were evaluated. Nanopore sequencing demonstrated an overall sensitivity of 91.7%, specificity of 85.3%, PPV of 95.1%, NPV of 76.4%, and AUC of 0.88. Notably, the overall diagnostic accuracy of nanopore sequencing was significantly higher than that of (MTB) culture technique.
Nanopore sequencing exhibited satisfactory overall diagnostic accuracy for smear-negative PTB, regardless of MTB culture status. Therefore, if conditions permit, nanopore sequencing is recommended as a diagnostic method for smear-negative PTB.
本研究旨在评估纳米孔测序技术在使用涂片阴性临床标本诊断肺结核（PTB）方面的疗效。
我们通过回顾患者病历进行了一项回顾性研究，以评估纳米孔测序作为涂片阴性PTB诊断工具的准确性。与临床诊断相比，我们确定了纳米孔测序的敏感性、特异性、阳性预测值（PPV）、阴性预测值（NPV）和曲线下面积（AUC）。
共评估了647例患者。纳米孔测序的总体敏感性为91.7%，特异性为85.3%，PPV为95.1%，NPV为76.4%，AUC为0.88。值得注意的是，纳米孔测序的总体诊断准确性显著高于结核分枝杆菌（MTB）培养技术。
无论MTB培养状态如何，纳米孔测序对涂片阴性PTB均表现出令人满意的总体诊断准确性。因此，如果条件允许，建议将纳米孔测序作为涂片阴性PTB的诊断方法。
