Arfman Tom, Zollet Valentina, van Es Nick, Bosch Floris T M, Nicolaes Gerry A F, Sorvillo Nicoletta, Voorberg Jan
Department of Molecular Hematology Sanquin Research and Landsteiner Laboratory Amsterdam The Netherlands.
Department for BioMedical Research (DBMR) University of Bern Bern Switzerland.
EJHaem. 2024 Jan 26;5(1):136-140. doi: 10.1002/jha2.825. eCollection 2024 Feb.
Neutrophil released peptidyl arginine deiminase 4 (PAD4) converts arginine residues on plasma proteins into citrulline. Here, we developed an assay to quantify citrullinated fibrinogen. We employed a biotin-conjugated phenylglyoxal (biotin-phenylglyoxal (PG)) compound that selectively labels citrulline. Patient samples were derived from a multicenter prospective cohort study that aimed to identify cancer patients at high risk for venous thromboembolism (VTE). Our data show that cancer patients have higher (median 2-fold increased) citrullinated fibrinogen levels when compared to normal human plasma and a cohort of healthy donors. Our results show that citrullination of fibrinogen is a common posttranslational modification in patients with cancer.
中性粒细胞释放的肽基精氨酸脱亚氨酶4（PAD4）将血浆蛋白上的精氨酸残基转化为瓜氨酸。在此，我们开发了一种测定瓜氨酸化纤维蛋白原的方法。我们使用了一种生物素偶联的苯乙二醛（生物素 - 苯乙二醛（PG））化合物，该化合物可选择性标记瓜氨酸。患者样本来自一项多中心前瞻性队列研究，该研究旨在识别静脉血栓栓塞（VTE）高风险的癌症患者。我们的数据表明，与正常人血浆和一组健康供体相比，癌症患者的瓜氨酸化纤维蛋白原水平更高（中位数增加2倍）。我们的结果表明，纤维蛋白原的瓜氨酸化是癌症患者中常见的翻译后修饰。
