IL28B 基因在巴基斯坦开伯尔-普赫图赫瓦省对达卡他韦和索非布韦耐药的 HCV 患者中的预测潜力。

Predictive Potential Of Il28b Gene In Hcv Patients, Resistant To Daclatasvir And Sofosbuvir In KPK Population.

机构信息

Department of Pathology, Peshawar Medical College, Peshawar, Pakistan.

出版信息

J Ayub Med Coll Abbottabad. 2023 Oct-Dec;35(4):523-529. doi: 10.55519/JAMC-04-11760.

DOI:10.55519/JAMC-04-11760

PMID:38406928

Abstract

BACKGROUND

Recently various combinations of direct acting antivirals (DAAs) have been tried successfully. The Sofosbuvir + Daclatasvir combination has been used with promising results. Recently, resistance has been noticed against DAAs. Therefore, polymorphism at particular sites in the interleukin 28B gene are under study to find possible association with resistance. This study was aimed at finding out any association of SNPs rs8099917 and rs12979860 (IL28B gene) with response and resistance to treatment in HCV genotype 3 patients in Khyber Pakhtunkhwa.

METHODS

This cross sectional, Analytical study was conducted at Gastroenterology/hepatology OPD of Prime Teaching Hospital, Peshawar Medical College. Collected Samples were stored at -20o C in PCR Lab of the College. DNA extraction and genotyping was carried out at BJ Molecular Biology Lab in Rawalpindi. Data was analyzed by using SPSS version 21. Chi-Square Test was used to see the statistically significant differences between rs8099917 T/G and rs12979860 T/C model.

RESULTS

In the IL28-B gene, single nucleotide polymorphism at rs12979860 T/C model, we observed that there are 37.5% CC homozygous, 12.5% TT homozygous and 50% CT heterozygous genotypes in resistant patients and 42.85% CC homozygous, 28.57% TT and 28.57% CT genotype in responder group. In rs12979860 T/C model, genotype of IL28-B in the responder and resistant group significantly varies at p-value =0.00572.

CONCLUSION

We conclude that in SNP at rs12979860, CC genotype is associated with clearance of HCV, while CT genotype was more prevalent in the resistant group and associated with chronicity.

摘要

背景

最近，各种直接作用抗病毒药物（DAAs）的组合已被成功尝试。索非布韦+达拉他韦的组合已取得了令人鼓舞的效果。最近，已经注意到对 DAA 的耐药性。因此，白细胞介素 28B 基因中特定位点的多态性正在研究中，以寻找与耐药性可能的关联。本研究旨在探讨 HCV 基因型 3 患者中 IL28B 基因的 SNPs rs8099917 和 rs12979860 与治疗反应和耐药性的任何关联。

方法

这是一项在白沙瓦医科大学的胃肠病学/肝病学 OPD 进行的横断面、分析性研究。收集的样本储存在学院的 PCR 实验室中-20o C。DNA 提取和基因分型在拉瓦尔品第的 BJ 分子生物学实验室进行。数据使用 SPSS 版本 21 进行分析。卡方检验用于观察 rs8099917 T/G 和 rs12979860 T/C 模型之间的统计学显著差异。

结果

在 IL28-B 基因中，rs12979860 T/C 模型的单核苷酸多态性，我们观察到耐药患者中有 37.5% CC 纯合子、12.5% TT 纯合子和 50% CT 杂合子基因型，而在应答组中有 42.85% CC 纯合子、28.57% TT 和 28.57% CT 基因型。在 rs12979860 T/C 模型中，IL28-B 的基因型在应答组和耐药组之间在 p 值=0.00572 时显著不同。