USDA-ARS, Tropical Crop and Commodity Protection Research Unit, Daniel K Inouye US Pacific Basin Agriculture Research Center, Hilo, HI, 96720, USA.
College of Tropical Agriculture and Human Resources, Komohana Research and Extension Center, University of Hawai'i, Hilo, HI, 96720, USA.
Microb Ecol. 2024 Feb 26;87(1):46. doi: 10.1007/s00248-024-02352-9.
Bacterial symbionts are crucial to the biology of Bactrocera dorsalis. With larval diet (fruit host) being a key factor that determines microbiome composition and with B. dorsalis using more than 400 fruits as hosts, it is unclear if certain bacterial symbionts are preserved and are passed on to B. dorsalis progenies despite changes in larval diet. Here, we conducted a fly rearing experiment to characterize diet-induced changes in the microbiome of female B. dorsalis. In order to explicitly investigate the impacts of larval diet on the microbiome, including potential stable bacterial constituents of B. dorsalis, we performed 16S rRNA sequencing on the gut tissues of teneral female flies reared from four different host fruits (guava, mango, papaya, and rose apple) infested using a single cohort of wild B. dorsalis that emerged from tropical almond (mother flies). Although B. dorsalis-associated microbiota were predominantly shaped by the larval diet, some major bacterial species from the mother flies were retained in progenies raised on different larval diets. With some variation, Klebsiella (ASV 1 and 2), Morganella (ASV 3), and Providencia (ASV 6) were the major bacterial symbionts that were stable and made up 0.1-80% of the gut and ovipositor microbiome of female teneral flies reared on different host fruits. Our results suggest that certain groups of bacteria are stably associated with female B. dorsalis across larval diets. These findings provide a basis for unexplored research on symbiotic bacterial function in B. dorsalis and may aid in the development of novel management techniques against this devastating pest of horticultural importance.
细菌共生体对桔小实蝇的生物学至关重要。幼虫的饮食（水果宿主）是决定微生物组组成的关键因素，而桔小实蝇使用超过 400 种水果作为宿主，因此尚不清楚某些细菌共生体是否被保留下来，并传递给桔小实蝇的后代，尽管幼虫的饮食发生了变化。在这里，我们进行了一项蝇类饲养实验，以描述雌性桔小实蝇的微生物组因饮食而发生的变化。为了明确研究幼虫饮食对微生物组的影响，包括桔小实蝇潜在的稳定细菌组成部分，我们对从四种不同的宿主水果（番石榴、芒果、木瓜和玫瑰苹果）中饲养的、刚羽化的雌性桔小实蝇的肠道组织进行了 16S rRNA 测序，这些桔小实蝇是使用来自热带杏仁（母蝇）的单一群体野生桔小实蝇孵化的。尽管桔小实蝇相关的微生物组主要受幼虫饮食的影响，但在不同的幼虫饮食中饲养的后代中仍保留了一些来自母蝇的主要细菌种类。在一定程度上，克雷伯氏菌（ASV1 和 2）、摩根菌（ASV3）和普罗威登斯菌（ASV6）是稳定的主要细菌共生体，它们构成了在不同宿主水果上饲养的雌性刚羽化的桔小实蝇的肠道和产卵器微生物组的 0.1-80%。我们的研究结果表明，某些细菌群在桔小实蝇幼虫的饮食中稳定地与其相关联。这些发现为探索桔小实蝇共生细菌功能提供了依据，并可能有助于开发针对这种具有破坏性的园艺害虫的新型管理技术。
