de Duve Institute, Université catholique de Louvain, Brussels, Belgium.
Methods Mol Biol. 2024;2849:45-54. doi: 10.1007/7651_2024_513.
The mammalian integumentary system, including skin and its appendages, serves as a protective barrier for the body. During development, skin epidermis undergoes rapid cell division and differentiation to form multiple stratified layers of keratinocytes. Concurrently the epidermis also gives rise to hair follicles that invaginate into the dermis. In adult skin, the hair follicle undergoes cyclic regeneration fueled by hair follicle stem cells located in the bulge. Three-dimensional and high-resolution imaging of these structures using whole-mount immunofluorescent staining allows to better characterize epidermal progenitors and stem cells.
哺乳动物的表皮系统，包括皮肤及其附属物，是身体的一道保护屏障。在发育过程中，皮肤表皮经历快速的细胞分裂和分化，形成多层角质形成细胞。同时，表皮还产生了向内凹陷到真皮中的毛囊。在成年皮肤中，毛囊通过位于隆突处的毛囊干细胞的周期性再生来维持。使用全组织免疫荧光染色对这些结构进行三维和高分辨率成像，可以更好地对表皮祖细胞和干细胞进行特征描述。
