Department of Pulmonary and Critical Care Medicine, Shengjing Hospital of China Medical University, Shenyang 110004, China.
Department of Gastroenterology Medicine, Shengjing Hospital of China Medical University, Shenyang 110004, China.
J Proteome Res. 2024 Apr 5;23(4):1232-1248. doi: 10.1021/acs.jproteome.3c00664. Epub 2024 Feb 26.
The aim of this study was to identify serum diagnostic biomarkers associated with the severity of obstructive sleep apnea (OSA) during pregnancy. Differentially expressed proteins (DEPs) were identified in the control (C), mild (O), and moderate (MO) OSA groups ( = 3 in each group). Bioinformatics analysis was conducted to identify the underlying functions, pathways, and networks of the proteins. Receiver operating characteristic curves were used to assess the diagnostic value of the identified DEPs. The enzyme-linked immunoassay was performed to detect serum levels of the complement C1r subcomponent (C1R) and alpha-2-macroglobulin (A2M) in 79 pregnant women with OSA (mild OSA [ = 32]; moderate OSA [ = 29], and severe OSA [ = 18]) and 65 healthy pregnant women without OSA. Pearson's correlation analysis was conducted to analyze the correlation between C1R and A2M levels and OSA clinicopathological factors. In total, 141 DEPs, 29 DEPs, and 103 DEPs were identified in the three groups (i.e., the mild OSA vs control group, the moderate OSA vs mild apnea group, and the moderate OSA vs control group, respectively). C1R and A2M were identified as continuously up-regulated proteins, and the levels of C1R and A2M were associated with OSA severity. C1R and A2M were found to be correlated with body mass index, systolic blood pressure, apnea-hypopnea index, oxygen desaturation index, time with saturation below 90%, and lowest SaO. Adverse maternal and neonatal outcomes were observed in pregnant women with OSA. C1R and A2M have been identified as diagnostic biomarkers and are associated with the severity of OSA during pregnancy.
本研究旨在确定与妊娠期阻塞性睡眠呼吸暂停（OSA）严重程度相关的血清诊断生物标志物。在对照组（C）、轻度（O）和中度（MO）OSA 组中分别鉴定出差异表达蛋白（DEPs）（每组 3 个）。进行生物信息学分析以鉴定蛋白质的潜在功能、途径和网络。使用受试者工作特征曲线评估所鉴定的 DEPs 的诊断价值。采用酶联免疫吸附试验检测 79 例 OSA 孕妇（轻度 OSA [=32]；中度 OSA [=29]，重度 OSA [=18]）和 65 例无 OSA 的健康孕妇的血清补体 C1r 亚成分（C1R）和α-2-巨球蛋白（A2M）水平。进行 Pearson 相关性分析以分析 C1R 和 A2M 水平与 OSA 临床病理因素之间的相关性。在三组中（即轻度 OSA 与对照组、中度 OSA 与轻度 OSA 组、中度 OSA 与对照组）分别鉴定出 141 个 DEPs、29 个 DEPs 和 103 个 DEPs。C1R 和 A2M 被鉴定为连续上调蛋白，且 C1R 和 A2M 水平与 OSA 严重程度相关。发现 C1R 和 A2M 与体重指数、收缩压、呼吸暂停低通气指数、氧减指数、饱和度低于 90%的时间和最低 SaO 相关。OSA 孕妇出现不良母婴结局。C1R 和 A2M 已被确定为诊断生物标志物，与妊娠期 OSA 的严重程度相关。
