Martin N F, Gaasterland D E, Rodrigues M M, Thomas G, Cummins C E
Ophthalmology. 1985 Oct;92(10):1382-6. doi: 10.1016/s0161-6420(85)33860-5.
Laser pulses were focused within 1 mm of the rhesus monkey corneal endothelium using the Coherent Model 9900 laser at energies of 3, 6, 9, and 12 mJ. Sixteen slightly off-axis pulses were applied with no contact lens on the eye. Corneal damage was studied clinically and by scanning electron microscopy. Q-switched pulses of 12 mJ or less are not likely to damage the cornea if focused more than 0.75 mm from the endothelium. The retrocorneal focal distance for a 50% incidence of endothelial damage for 6, 9, and 12 mJ pulses was found to be less than 0.5 mm. For 3 mJ pulses, it was less than 0.25 mm. The severe early effect of suprathreshold pulses is edema of endothelial cells in a 0.2- to 0.6-mm diameter circular zone surrounding a small central pit through Descemet's membrane. At two months, irregular enlargement of endothelial cells surrounding and partially covering the persistent pit exists at sites of severe damage. The extent of the longer-term change is proportional to the severity of the original insult, but in no case was there clinically significant, persistent damage in the healed corneas.
使用相干9900型激光，以3、6、9和12毫焦的能量将激光脉冲聚焦在恒河猴角膜内皮1毫米范围内。在眼睛上不戴隐形眼镜的情况下施加16个略微偏离轴线的脉冲。通过临床检查和扫描电子显微镜研究角膜损伤情况。如果聚焦点距离内皮超过0.75毫米，12毫焦及以下的调Q脉冲不太可能损伤角膜。对于6、9和12毫焦的脉冲，发现内皮损伤发生率为50%时的角膜后焦距离小于0.5毫米。对于3毫焦的脉冲，该距离小于0.25毫米。阈上脉冲的严重早期效应是在围绕通过Descemet膜的小中央凹坑的直径0.2至0.6毫米的圆形区域内内皮细胞水肿。在两个月时，严重损伤部位存在围绕并部分覆盖持续凹坑的内皮细胞不规则增大。长期变化的程度与原始损伤的严重程度成正比，但在任何情况下，愈合后的角膜都没有临床上显著的持续性损伤。
