横纹钝头蛇的多倍体化：同工酶测序的证据。

Polyploidization of Indotyphlops braminus: evidence from isoform-sequencing.

机构信息

School of Life Sciences, Guizhou Normal University, 550025, Guiyang, Guizhou, China.

Department of Bioinformatics, DNA Stories Bioinformatics Center, 610000, Chengdu, China.

出版信息

BMC Genom Data. 2024 Feb 26;25(1):23. doi: 10.1186/s12863-024-01208-y.

DOI:10.1186/s12863-024-01208-y

PMID:38408920

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10895795/

Abstract

BACKGROUND

Indotyphlops braminus, the only known triploid parthenogenetic snake, is a compelling species for revealing the mechanism of polyploid emergence in vertebrates.

METHODS

In this study, we applied PacBio isoform sequencing technology to generate the first full-length transcriptome of I. braminus, aiming to improve the understanding of the molecular characteristics of this species.

RESULTS

A total of 51,849 nonredundant full-length transcript assemblies (with an N50 length of 2980 bp) from I. braminus were generated and fully annotated using various gene function databases. Our analysis provides preliminary evidence supporting a recent genome duplication event in I. braminus. Phylogenetic analysis indicated that the divergence of I. braminus subgenomes occurred approximately 11.5 ~ 15 million years ago (Mya). The full-length transcript resource generated as part of this research will facilitate transcriptome analysis and genomic evolution studies in the future.

摘要

背景

蚓蜥，唯一已知的三倍体孤雌生殖蛇，是揭示脊椎动物多倍体出现机制的一个引人注目的物种。

方法

本研究应用 PacBio 异构体测序技术，生成蚓蜥的首个全长转录组，旨在提高对该物种分子特征的理解。

结果

共生成了 51849 条非冗余的全长转录本组装体（N50 长度为 2980 bp），并使用各种基因功能数据库进行了全面注释。我们的分析初步支持蚓蜥最近发生了基因组加倍事件。系统发育分析表明，蚓蜥亚基因组的分化发生在大约 1150 万至 1500 万年前。作为本研究的一部分生成的全长转录本资源，将有助于未来的转录组分析和基因组进化研究。