利用 Cas13a 对 RNA G-四链体的切割进行无扩增的 RNA 检测。
Leveraging Cas13a's -cleavage on RNA G-quadruplexes for amplification-free RNA detection.
机构信息
State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, Key Laboratory of Magnetic Resonance in Biological Systems, National Center for Magnetic Resonance in Wuhan, Wuhan Institute of Physics and Mathematics, Innovation Academy for Precision Measurement Science and Technology-Wuhan National Laboratory for Optoelectronics, Chinese Academy of Sciences, Wuhan 430071, China.
University of Chinese Academy of Sciences, Beijing 100049, China.
出版信息
Chem Commun (Camb). 2024 Mar 14;60(23):3166-3169. doi: 10.1039/d3cc06238d.
In this study, we investigated Cas13a's efficacy in -cleaving RNA G-quadruplexes (rG4s) as an alternative to ssRNA reporters in CRISPR-Cas13a diagnostics. Our findings demonstrate enhanced efficiency due to the structural arrangement of rG4s. Implementing a simplified CRISPR-Cas13a system based on rG4, we identified SARS-CoV-2 infections in 25 patient samples within 1 hour without target pre-amplification.
在这项研究中，我们研究了 Cas13a 在 - 切割 RNA G-四链体 (rG4) 方面的功效，作为 CRISPR-Cas13a 诊断中 ssRNA 报告物的替代物。我们的研究结果表明，由于 rG4 的结构排列，效率得到了提高。我们在基于 rG4 的简化 CRISPR-Cas13a 系统中，在 1 小时内无需目标预扩增即可在 25 个患者样本中识别 SARS-CoV-2 感染。
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