Smith D B, Inglis S C
EMBO J. 1985 Sep;4(9):2313-9. doi: 10.1002/j.1460-2075.1985.tb03932.x.
The influenza virus NS2 mRNA is generated through processing by cellular enzymes of a transcript (the NS1 mRNA) of virion RNA segment 8. Production of this mRNA is altered in cells infected with a mutant of influenza A (fowl plague) virus. The proportion of segment 8 transcripts which accumulated in a spliced form was found to be considerably lower in mutant virus-infected cells than in cells infected with wild-type virus, and the amplification in production of NS2 mRNA relative to that of the NS1 mRNA, which normally occurs during infection with wild-type virus, was not observed with the mutant. The NS1 mRNA specified by the mutant virus has unaltered splice recognition sites and was apparently processed normally during a mixed infection with a strain of virus which is wild-type for production of NS2 mRNA. These results suggest that the production of NS2 mRNA is regulated by virus-specific products; these products may act by increasing the efficiency of splicing of NS1 mRNA.
流感病毒NS2 mRNA是通过病毒粒子RNA片段8的转录本（NS1 mRNA）经细胞酶加工产生的。在感染甲型流感（禽瘟）病毒突变体的细胞中，这种mRNA的产生会发生改变。发现在突变病毒感染的细胞中，以剪接形式积累的片段8转录本的比例比野生型病毒感染的细胞中要低得多，并且在突变体中未观察到相对于NS1 mRNA产量通常在野生型病毒感染期间发生的NS2 mRNA产量的增加。突变病毒指定的NS1 mRNA具有未改变的剪接识别位点，并且在与NS2 mRNA产生为野生型的病毒株混合感染期间显然正常加工。这些结果表明，NS2 mRNA的产生受病毒特异性产物调节；这些产物可能通过提高NS1 mRNA的剪接效率起作用。
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