Lamb R A, Lai C J, Choppin P W
Proc Natl Acad Sci U S A. 1981 Jul;78(7):4170-4. doi: 10.1073/pnas.78.7.4170.
RNA segment 7 of the influenza A virus genome codes for at least two proteins, M1 and M2, which are synthesized from separate mRNA species. Sequence analysis of the M2 mRNA has shown that it contains an interrupted sequence of 689 nucleotides. The approximately 51 virus-specific nucleotides comprising the 5'-end leader sequence of the M2 mRNA are the same as those found at the 5' end of the colinear M1 mRNA. Following the leader sequence of the M2 mRNA, where is a 271-nucleotide body region that is 3' coterminal with the M1 mRNA. Another small potential mRNA (mRNA3) related to RNA segment 7 has been found. mRNA3 has a leader sequence of approximately 11 virus-specific nucleotides that are the same as the 5' end of the M1 and M2 mRNAs, followed by an interrupted sequence of 729 nucleotides, and then a body region of approximately 271 nucleotides that is the same as that of the M2 mRNA. The nucleotide sequences found at the junctions of the interrupted sequences in M2 mRNA and mRNA3 are similar to those found at the splicing points of intervening sequences in eukaryotic mRNAs. In addition, both mRNAs contain 10-15 heterogeneous nonviral nucleotides at their 5' ends that appear to be derived from cellular RNAs used for priming the transcription of viral RNAs. Because the 5'-end sequences of the M1 mRNA and the M2 mRNA are the same and share the 5'-proximal initiation codon for protein synthesis, the first nine amino acids would be the same in the M1 and M2 protein and then the sequences would diverge. The approximately 271-nucleotide body region of the M2 mRNA can be translated in the +1 reading frame, and the sequence indicates that M1 and M2 overlap by 14 amino acids. The coding potential of the mRNA3 is for only nine amino acids, and these would be identical to the COOH-terminal region of the membrane protein (M1).
甲型流感病毒基因组的RNA片段7编码至少两种蛋白质，即M1和M2，它们由不同的mRNA种类合成。M2 mRNA的序列分析表明，它包含一个689个核苷酸的间断序列。构成M2 mRNA 5'端前导序列的大约51个病毒特异性核苷酸与共线性M1 mRNA 5'端的核苷酸相同。在M2 mRNA的前导序列之后，有一个271个核苷酸的主体区域，它与M1 mRNA 3'端共末端。已发现另一种与RNA片段7相关的小的潜在mRNA（mRNA3）。mRNA3有一个大约11个病毒特异性核苷酸的前导序列，与M1和M2 mRNA的5'端相同，随后是一个729个核苷酸的间断序列，然后是一个大约271个核苷酸的主体区域，与M2 mRNA的主体区域相同。在M2 mRNA和mRNA3间断序列连接处发现的核苷酸序列与真核mRNA中居间序列的剪接位点处发现的序列相似。此外，两种mRNA在其5'端都含有10 - 15个异质非病毒核苷酸，这些核苷酸似乎来自用于引发病毒RNA转录的细胞RNA。由于M1 mRNA和M2 mRNA的5'端序列相同且共享蛋白质合成的5'近端起始密码子，M1和M2蛋白的前九个氨基酸将相同，然后序列会 diverge。M2 mRNA大约271个核苷酸的主体区域可以在+1阅读框中翻译，序列表明M1和M2重叠14个氨基酸。mRNA3的编码潜能仅为九个氨基酸，且这些氨基酸与膜蛋白（M1）的COOH末端区域相同。
