Rothera M A, Wehrli S, Cook J M
J Nat Prod. 1985 Sep-Oct;48(5):802-8. doi: 10.1021/np50041a015.
The MeOH extract of Corydalis clarkei Prain yielded two tetrahydroprotoberberine alkaloids that bear the unusual 1,2- and 9, 10-tetraoxygenated substitution pattern observed previously only in caseanidine . The alkaloids have been identified as caseanidine (1) and the new alkaloid, clarkeanidine (2). The 1H- and 13C-nmr shifts of both alkaloids have been assigned unambiguously using 2-D nmr. The elucidation of the structure of 2 was achieved by comparison of the 1H- and 13C-nmr spectra of 2 with those of caseanidine coupled with additional spectroscopic evidence.
紫堇（Corydalis clarkei Prain）的甲醇提取物产生了两种四氢原小檗碱生物碱，它们具有不寻常的1,2-和9,10-四氧化取代模式，这种模式以前仅在酪胺idine中观察到。这些生物碱已被鉴定为酪胺idine（1）和新生物碱，紫堇胺idine（2）。使用二维核磁共振明确归属了两种生物碱的1H和13C核磁共振位移。通过将2的1H和13C核磁共振光谱与酪胺idine的光谱进行比较，并结合其他光谱证据，确定了2的结构。
