Key Laboratory of Agricultural Microbiomics and Precision Application (MARA), Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Key Laboratory of Agricultural Microbiome (MARA), State Key Laboratory of Applied Microbiology Southern China, Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou 510070, People's Republic of China.
Key Laboratory of Agricultural Microbiomics and Precision Application (MARA), Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Key Laboratory of Agricultural Microbiome (MARA), State Key Laboratory of Applied Microbiology Southern China, Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou 510070, People's Republic of China.
Int J Biol Macromol. 2024 Apr;264(Pt 1):130400. doi: 10.1016/j.ijbiomac.2024.130400. Epub 2024 Feb 25.
The transcription factor complex INO2 and INO4 in Saccharomyces cerevisiae plays a vital role in lipid biosynthesis by activating multiple genes in the biosynthetic pathways of phospholipid, fatty acid, and sterol. Previous studies have reported conflicting results regarding the effects of ino2 and ino4 gene expression levels on target chemicals. Therefore, this study aimed to examine the influence of different ino2 and ino4 expression levels on carotenoid production (e.g., lycopene), which shares a common precursor, acetyl-CoA, with lipid metabolism. Surprisingly, 2.6- and 1.8-fold increase in lycopene yield in the ino2 and ino4 deletion strains were found, respectively. In contrast, ino2 overexpression did not promote lycopene accumulation. Additionally, there was a decrease in intracellular free fatty acids in the ino2 deletion strain. Comparative transcriptome analysis revealed a significant downregulation of genes related to lipid biosynthesis in the ino2 deletion strain. To our knowledge, this is the first report showing that deletion of transcription factor genes ino2 and ino4 can facilitate lycopene accumulation. These findings hold significant implications for the development of metabolically engineered S. cerevisiae with enhanced carotenoid production.
酿酒酵母中的转录因子复合物 INO2 和 INO4 通过激活磷脂、脂肪酸和固醇生物合成途径中的多个基因，在脂质生物合成中发挥着至关重要的作用。先前的研究报告称，INO2 和 INO4 基因表达水平对目标化学物质的影响存在矛盾。因此，本研究旨在研究不同 INO2 和 INO4 表达水平对类胡萝卜素（如番茄红素）产生的影响，因为类胡萝卜素与脂质代谢共享一个共同的前体乙酰辅酶 A。令人惊讶的是，INO2 和 INO4 缺失菌株中的番茄红素产量分别增加了 2.6 倍和 1.8 倍。相比之下，INO2 的过表达并没有促进番茄红素的积累。此外，INO2 缺失菌株中的细胞内游离脂肪酸减少。比较转录组分析显示，INO2 缺失菌株中与脂质生物合成相关的基因显著下调。据我们所知，这是首次报道转录因子基因 INO2 和 INO4 的缺失可以促进番茄红素的积累。这些发现对开发具有增强类胡萝卜素生产能力的代谢工程酿酒酵母具有重要意义。
