Department of Gastroenterology and Hepatology, Nagoya University Graduate School of Medicine, 65 Tsuruma-cho, Showa-ku, Nagoya 466-8550, Japan.
Department of Gastroenterology, The University of Tokyo Hospital, 7-3-1, Hongo, Bunkyo-ku, Tokyo 113-8655, Japan.
Dig Liver Dis. 2024 Jun;56(6):941-950. doi: 10.1016/j.dld.2024.01.212. Epub 2024 Feb 26.
Nonampullary duodenal epithelial tumors are rare, but their prevalence is increasing. Various gastrointestinal cancers have been associated with microbiomes. We evaluated the characteristics of the salivary and duodenal microbiomes of patients with nonampullary duodenal epithelial tumors.
Saliva and biopsy samples from the duodenal bulb and descending portion were obtained from 15 patients with nonampullary duodenal epithelial tumors and 10 controls. Next-generation sequencing was performed to identify bacteria for comparison.
Saliva samples had higher Amplicon Sequence Variants (ASVs) and more observed species than duodenal samples. Saliva samples from patients with nonampullary duodenal epithelial tumor were dominated by Bacteroidetes and Prevotella, whereas Proteobacteria and Neisseria were dominant in the control samples. The relative abundance of bacteria was higher in patients with nonampullary duodenal epithelial tumors. Most bacteria were classified as bacteria of oral origin. Oribacterium and Stomatobaculum were significantly higher in the saliva, duodenal bulb, and descending portion of patients with nonampullary duodenal epithelial tumors.
Patients with nonampullary duodenal epithelial tumors had different salivary and duodenal microbiomes than controls. Bacteria types differed between groups at each site, and most bacteria of oral origin were more abundant in patients with nonampullary duodenal epithelial tumors.
非壶腹十二指肠上皮肿瘤较为罕见，但发病率呈上升趋势。各种胃肠道癌症都与微生物群有关。我们评估了非壶腹十二指肠上皮肿瘤患者唾液和十二指肠微生物群的特征。
从 15 名非壶腹十二指肠上皮肿瘤患者和 10 名对照者的十二指肠球部和降部采集唾液和活检样本。进行下一代测序以鉴定细菌进行比较。
唾液样本的扩增子序列变异体（ASV）和观察到的物种比十二指肠样本多。非壶腹十二指肠上皮肿瘤患者的唾液样本以拟杆菌门和普雷沃氏菌属为主，而对照组以变形菌门和奈瑟菌属为主。非壶腹十二指肠上皮肿瘤患者的细菌相对丰度较高。大多数细菌被归类为口腔来源的细菌。非壶腹十二指肠上皮肿瘤患者的唾液、十二指肠球部和降部的奥里杆菌属和齿龈双歧杆菌属明显升高。
非壶腹十二指肠上皮肿瘤患者的唾液和十二指肠微生物群与对照组不同。每个部位的细菌类型在组间存在差异，大多数口腔来源的细菌在非壶腹十二指肠上皮肿瘤患者中更为丰富。
