Stoker M, Perryman M
J Cell Sci. 1985 Aug;77:209-23. doi: 10.1242/jcs.77.1.209.
Medium conditioned by human embryo fibroblasts breaks structural junctions between several types of epithelial cells, leading to separation and scattering of the cells. An assay developed in MDCK cells shows activity up to a dilution of at least 1 in 64, equivalent to less than 100 ng of total protein. The activity is non-dialysable, heat-labile, and sensitive to trypsin, and it is assumed to be due to one or more proteins. After addition of the factor, separation of MDCK cells begins in about 15 min and is complete in 10 h. It increases migration of MDCK cells into wounds, and causes collapse of domes. Locomotion of isolated cells is not enhanced, but cell shape is affected by local membrane movement. Under the culture conditions used the factor, or an associated protein, causes a weak inhibition of cell growth without cytotoxic activity. The scattering factor has not been purified, nor has a physiological role been identified, but it might be concerned in the mobilization of epithelial cells.
人胚胎成纤维细胞条件培养液可破坏几种上皮细胞间的结构连接，导致细胞分离和分散。在MDCK细胞中开发的一种检测方法显示，其活性在至少1/64的稀释度下仍存在，相当于总蛋白量少于100 ng。该活性不可透析、对热不稳定且对胰蛋白酶敏感，推测其由一种或多种蛋白质引起。添加该因子后，MDCK细胞在约15分钟开始分离，10小时内完成。它可增加MDCK细胞向伤口处的迁移，并导致圆顶结构塌陷。孤立细胞的运动未增强，但细胞形状受局部膜运动影响。在所使用的培养条件下，该因子或相关蛋白会对细胞生长产生微弱抑制，但无细胞毒性活性。散射因子尚未纯化，其生理作用也未明确，但可能与上皮细胞的移动有关。
