Vala Dushyantsinh, Mehta Tarang, Arjun Gadakh Mahesh, Patel Mimansha, Mondal Animesh, Gupta Bharti
Department of Oral and Maxillofacial Pathology and Oral Microbiology, Government Dental College and Hospital, Jamnagar, India.
Department of Oral & Maxillofacial Pathology and Oral Microbiology, Narsinhbhai Patel Dental College & Hospital, Sankalchand Patel University, Gujarat, India.
Bioinformation. 2023 Dec 31;19(13):1405-1410. doi: 10.6026/973206300191405. eCollection 2023.
The expression analysis of cyclin D1, Ki-67, MCM3 and MCM2 in oral squamous cell carcinoma to identify biomarkers is of interest. 45 formalin-fixed paraffin embedded tissue blocks collected from archives of Department of Oral and Maxillofacial Pathology and Oral Microbiology, Government Dental College and Hospital, Jamnagar, India were subjected to a retrospective cross-sectional immuno-histo-chemistry examination. 30 blocks of OSCC with histological diagnosis have 15 tissue blocks of well-differentiated oral carcinoma and 15 tissue blocks of moderately-differentiated oral carcinoma. 15 specimens of normal oral mucosa (NM) were also examined for comparison. In each of the categories, the immuno-histo-chemistry expression of cyclin D1, MCM 3, MCM 2, and ki67 was studied. Data shows that cyclin D1, Ki-67, MCM3 and MCM2 effectively indicate cellular proliferation for consideration as potential biomarkers of oral squamous cell carcinoma.
对细胞周期蛋白D1、Ki-67、微小染色体维持蛋白3(MCM3)和微小染色体维持蛋白2(MCM2)在口腔鳞状细胞癌中的表达进行分析以鉴定生物标志物具有重要意义。从印度贾姆讷格尔政府牙科学院和医院口腔颌面病理学与口腔微生物学系档案中收集的45个福尔马林固定石蜡包埋组织块进行了回顾性横断面免疫组织化学检查。30个经组织学诊断的口腔鳞状细胞癌组织块中有15个高分化口腔癌组织块和15个中分化口腔癌组织块。还检查了15个正常口腔黏膜(NM)标本用于比较。在每个类别中,研究了细胞周期蛋白D1、MCM 3、MCM 2和ki67的免疫组织化学表达。数据表明,细胞周期蛋白D1、Ki-67、MCM3和MCM2可有效指示细胞增殖,有望作为口腔鳞状细胞癌的潜在生物标志物。