Rezazadeh Fahimeh, Ebrahimi Rahele, Andisheh-Tadbir Azadeh, Ashraf Mohammad Javad, Khademi Bijan
Oral and Dental Disease Research Center, Dept. of Oral & Maxillofacial Medicine, School of Dentistry, Shiraz University of Medical Sciences, Shiraz, Iran.
Dept. of Oral & Maxillofacial Medicine, School of Dentistry, Shahrekord University of Medical Sciences, Shahrekord, Iran.
J Dent (Shiraz). 2017 Sep;18(3):207-211.
Squamous cell carcinoma (SCC) is the most frequent oral cancer whose 5-year survival rate is 80% for early-detected lesions and nearly 30-50% for advanced lesions. Early detection of oral cancers and precancerous lesions can improve the patient's survival and decrease the morbidity.
This study aimed to evaluate and compare the Ki-67 and MCM3 expression in cytologic smear of oral SCC (OSCC).
We examined 48 oral brush biopsies including 28 OSCC and 20 healthy non-smoking samples. Immunocytochemistry staining was performed for Ki-67 and MCM3 by using an EnVision-labeled peroxidase system, and labeling index (LI) was calculated.
Out of 28 OSCC cases, 27(96.4%) cases contained MCM3 positive cells and 22(78%) cases contained Ki-67 positive cells. All normal mucosa were Ki-67 and MCM3 negative. MCM3 and Ki-67 LI were significantly higher in OSCC than normal mucosa (< 0.001). MCM3 LI was significantly higher than Ki-67 LI in OSCC group (< 0.001).
Immunocytologic evaluation of Ki-67 and MCM3 can be used for early detection of OSCC. Furthermore, MCM3 may be a more sensitive cytologic biomarker than Ki-67 in SCC patients.
鳞状细胞癌（SCC）是最常见的口腔癌，早期发现的病变5年生存率为80%，晚期病变的5年生存率接近30%-50%。早期发现口腔癌和癌前病变可提高患者生存率并降低发病率。
本研究旨在评估和比较口腔鳞状细胞癌（OSCC）细胞学涂片上Ki-67和MCM3的表达情况。
我们检查了48例口腔刷检活检样本，其中包括28例OSCC样本和20例健康非吸烟样本。采用EnVision标记过氧化物酶系统对Ki-67和MCM3进行免疫细胞化学染色，并计算标记指数（LI）。
在28例OSCC病例中，27例（96.4%）含有MCM3阳性细胞，22例（78%）含有Ki-67阳性细胞。所有正常黏膜的Ki-67和MCM3均为阴性。OSCC中MCM3和Ki-67的LI显著高于正常黏膜（<0.001）。在OSCC组中，MCM3的LI显著高于Ki-67的LI（<0.001）。
对Ki-67和MCM3进行免疫细胞学评估可用于OSCC的早期检测。此外，在SCC患者中，MCM3可能是比Ki-67更敏感的细胞学生物标志物。
