Department of Rehabilitation Medicine, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, China.
Department of Rehabilitation Medicine, Guangzhou First People's Hospital, School of Medicine, South China University of Technology.
Regen Med. 2024 Feb;19(2):93-102. doi: 10.2217/rme-2023-0222. Epub 2024 Feb 28.
This study aimed to explore the efficacy and optimal delivery time of human umbilical cord mesenchymal stem cells (hUC-MSCs) in treating collagenase-induced Achilles tendinopathy. Achilles tendinopathy in rats at early or advanced stages was induced by injecting collagenase I into bilateral Achilles tendons. A total of 28 injured rats were injected with a hUC-MSC solution or normal saline into bilateral tendons twice and sampled after 4 weeks for histological staining, gene expression analysis, transmission electron microscope assay and biomechanical testing analysis. The results revealed better histological performance and a larger collagen fiber diameter in the MSC group. mRNA expression of TNF-α, IL-1β and MMP-3 was lower after MSC transplantation. Early MSC delivery promoted collagen I and TIMP-3 synthesis, and strengthened tendon toughness. hUC-MSCs demonstrated a therapeutic effect in treating collagenase-induced Achilles tendinopathy, particularly in the early stage of tendinopathy.
本研究旨在探讨人脐带间充质干细胞（hUC-MSCs）治疗胶原酶诱导的跟腱腱病的疗效和最佳递送时间。通过向双侧跟腱注射胶原酶 I 诱导大鼠早期或晚期跟腱腱病。将 28 只受伤大鼠的双侧肌腱分别注射 hUC-MSC 溶液或生理盐水 2 次，4 周后取样进行组织学染色、基因表达分析、透射电镜检测和生物力学测试分析。结果显示 MSC 组组织学表现更好，胶原纤维直径更大。MSC 移植后 TNF-α、IL-1β 和 MMP-3 的 mRNA 表达降低。早期 MSC 递送促进胶原 I 和 TIMP-3 的合成，增强肌腱韧性。hUC-MSCs 对胶原酶诱导的跟腱腱病具有治疗作用，尤其是在腱病的早期阶段。
