Department of Orthopedics, Orthopedic Oncology Institute of PLA, Tangdu Hospital, Air Force Medical University, Xi'an, Shaanxi, China.
Lintong Rehabilitation and Convalescent Centre of PLA Joint Logistics Support Force, Xi'an, Shaanxi, China.
Am J Sports Med. 2023 Jun;51(7):1831-1843. doi: 10.1177/03635465231167860. Epub 2023 May 2.
Treatment of painful chronic tendinopathy is challenging, and there is an urgent need to develop new regenerative methods. Irreversible electroporation (IRE) can lead to localized cell ablation by electrical pulses and induce new cell and tissue growth. Previously, the authors' group reported that electroporation-ablated tendons fully regenerated.
To assess the efficiency of IRE in improving tendon healing using a collagenase-induced Achilles tendinopathy rat model.
Controlled laboratory study.
After screening for the IRE ablation parameters, a collagenase-induced Achilles tendinopathy rat model was used to assess the efficacy of IRE in improving tendon healing via biomechanical, behavioral, histological, and immunofluorescence assessments.
The experiments showed that the parameter of 875 V/cm 180 pulses could ablate most tenocytes, and apoptosis was the main type of death in vitro. In vivo, IRE promoted the healing process of chronic tendinopathy in the Achilles tendon of rats, based on biomechanical, behavioral, and histological assessments. Finally, immunofluorescence results revealed that IRE improved blood supply in the early stages of tendon repair and could potentially reduce neuropathic pain.
IRE enhanced tendon tissue healing in a rat model of collagenase-induced Achilles tendinopathy.
IRE may as a potential alternative treatment for tendinopathy in clinical usage.
治疗慢性疼痛性肌腱病具有挑战性，迫切需要开发新的再生方法。不可逆电穿孔（IRE）可以通过电脉冲导致局部细胞消融，并诱导新的细胞和组织生长。先前，作者小组报道了电穿孔消融的肌腱完全再生。
使用胶原酶诱导的跟腱病大鼠模型评估 IRE 改善肌腱愈合的效率。
对照实验室研究。
在筛选出 IRE 消融参数后，使用胶原酶诱导的跟腱病大鼠模型通过生物力学、行为学、组织学和免疫荧光评估评估 IRE 改善肌腱愈合的效果。
实验表明，875 V/cm 180 个脉冲的参数可以消融大多数肌腱细胞，体外主要的死亡类型是细胞凋亡。在体内，IRE 通过生物力学、行为学和组织学评估促进了大鼠跟腱慢性肌腱病的愈合过程。最后，免疫荧光结果显示，IRE 改善了肌腱修复早期的血液供应，并可能减轻神经病理性疼痛。
IRE 增强了胶原酶诱导的跟腱病大鼠模型中的肌腱组织愈合。
IRE 可能成为临床应用中肌腱病的一种潜在替代治疗方法。
