State Key Laboratory for Structural Chemistry of Unstable and Stable Species, Institute of Chemistry, Chinese Academy of Sciences, CAS Research/Education Center for Excellence in Molecular Sciences, Beijing 100190, China.
University of the Chinese Academy of Sciences, Beijing 100049, China.
Anal Chem. 2024 Mar 12;96(10):4314-4321. doi: 10.1021/acs.analchem.4c00298. Epub 2024 Feb 28.
Pathogen detection is growing in importance in the early stages of bacterial infection and treatment due to the significant morbidity and mortality associated with bloodstream infections. Although various diagnostic approaches for pathogen detection have been proposed, most of them are time-consuming, with insufficient sensitivity and limited specificity and multiplexing capability for clinical use. Here, we report a force-encoding DNA nanomachine for simultaneous and high-throughput detection of multiple pathogens in blood through force-induced remnant magnetization spectroscopy (FIRMS). The force-encoding DNA nanomachines coupled with DNA walkers enable analytical sensitivity down to a single bacterium via a cascade signal amplification strategy. More importantly, it allows for rapid and specific profiling of various pathogens directly in blood samples, without being affected by factors such as light color and solution properties. We expect that this magnetic sensing platform holds great promise for various applications in biomedical research and clinical diagnostics.
病原体检测在细菌感染和治疗的早期阶段变得越来越重要，因为血流感染与显著的发病率和死亡率相关。尽管已经提出了各种用于病原体检测的诊断方法，但它们大多数都很耗时，并且灵敏度不足，特异性和多重检测能力有限，无法在临床中应用。在这里，我们通过力诱导剩余磁化光谱（FIRMS）报告了一种用于通过力编码 DNA 纳米机器同时和高通量检测血液中多种病原体的方法。力编码 DNA 纳米机器与 DNA 行走器结合使用，通过级联信号放大策略，将分析灵敏度降低到单个细菌。更重要的是，它可以直接在血液样本中快速且特异性地分析各种病原体，而不受颜色和溶液性质等因素的影响。我们期望这个磁传感平台在生物医学研究和临床诊断中有很大的应用前景。
