Department of Pathology, School of Medical Sciences, Health Campus, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan, Malaysia.
Department of Pathology, Hospital of Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan, Malaysia.
Asian Pac J Cancer Prev. 2024 Feb 1;25(2):521-527. doi: 10.31557/APJCP.2024.25.2.521.
The study aimed to evaluate E6 and E7 oncoproteins of HPV16 and HPV18 expression in formalin - fixed paraffin embedded (FFPE) tissue in different grades of the cervical lesion and evaluate the potential use of E6 and E7 oncoproteins derived from HPV 16 and 18 as diagnostic protein biomarkers for triaging cervical lesions.
A total of 102 FFPE cervical tissues were collected from 2 tertiary hospitals and immunohistochemical reactivity staining of E6 and E7 oncoproteins of HPV16 and HPV18 were evaluated using immunoreactive scoring (IRS) system and analysed statistically.
The result showed an increased oncoprotein expression with the progression of cervical lesions. There is a statistically significant association between histology grade and HPV16/18-E6 expression (p = 0.028). However, there are no significant association of histological grade to HPV16-E7 immunoreactivity score (p = 0.264) and HPV18-E7 (p=0.080).
The immunohistochemical expression of HPV oncoproteins is a potential alternative diagnostic tool applicable in a low-resource laboratory setting. The advantage of the histochemical evaluation is that this method is simpler to apply and less expensive in comparison to in situ mRNA hybridization. Nevertheless, our study also found that antibodies against HPV that are commercially available suffer quite substantial specificity issues such as background staining and inconsistency between different batches. Hence, the utilization of antibody-based staining warrants stringent quality control.
本研究旨在评估 HPV16 和 HPV18 的 E6 和 E7 癌蛋白在不同宫颈病变分级的福尔马林固定石蜡包埋（FFPE）组织中的表达，并评估 HPV16 和 18 的 E6 和 E7 癌蛋白作为宫颈病变分流的诊断蛋白生物标志物的潜在用途。
从 2 家 3 级医院共收集了 102 例 FFPE 宫颈组织，使用免疫反应评分（IRS）系统评估 HPV16 和 HPV18 的 E6 和 E7 癌蛋白的免疫反应性，并进行统计学分析。
结果表明，随着宫颈病变的进展，癌蛋白表达增加。组织学分级与 HPV16/18-E6 表达之间存在统计学显著关联（p=0.028）。然而，HPV16-E7 免疫反应评分（p=0.264）和 HPV18-E7 与组织学分级之间无显著相关性（p=0.080）。
HPV 癌蛋白的免疫组织化学表达是一种潜在的替代诊断工具，适用于资源有限的实验室环境。与原位 mRNA 杂交相比，组织化学评估的优势在于该方法更简单且更经济。然而，我们的研究还发现，市售的针对 HPV 的抗体存在相当大的特异性问题，例如背景染色和不同批次之间的不一致性。因此，基于抗体的染色的利用需要严格的质量控制。
