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一种七性物种通过一种新的蛋白质复合物识别自我和非自我交配型。

A seven-sex species recognizes self and non-self mating-type via a novel protein complex.

机构信息

Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, China.

National Key Laboratory of Crop Genetic Improvement, Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan, China.

出版信息

Elife. 2024 Feb 28;13:RP93770. doi: 10.7554/eLife.93770.

DOI:10.7554/eLife.93770
PMID:38415774
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10901506/
Abstract

Although most species have two sexes, multisexual (or multi-mating type) species are also widespread. However, it is unclear how mating-type recognition is achieved at the molecular level in multisexual species. The unicellular ciliate has seven mating types, which are determined by the MTA and MTB proteins. In this study, we found that both proteins are essential for cells to send or receive complete mating-type information, and transmission of the mating-type signal requires both proteins to be expressed in the same cell. We found that MTA and MTB form a mating-type recognition complex that localizes to the plasma membrane, but not to the cilia. Stimulation experiments showed that the mating-type-specific regions of MTA and MTB mediate both self- and non-self-recognition, indicating that uses a dual approach to achieve mating-type recognition. Our results suggest that MTA and MTB form an elaborate multifunctional protein complex that can identify cells of both self and non-self mating types in order to inhibit or activate mating, respectively.

摘要

虽然大多数物种有两种性别，但多性别的（或多交配类型）物种也广泛存在。然而，在多性别的物种中，交配类型识别是如何在分子水平上实现的还不清楚。单细胞纤毛虫有七种交配类型，由 MTA 和 MTB 蛋白决定。在这项研究中，我们发现这两种蛋白对于细胞发送或接收完整的交配类型信息都是必不可少的，并且交配类型信号的传递需要两种蛋白在同一细胞中表达。我们发现 MTA 和 MTB 形成一个位于质膜上的交配类型识别复合物，但不在纤毛上。刺激实验表明，MTA 和 MTB 的交配类型特异性区域介导了自我和非自我识别，表明 使用了双重方法来实现交配类型识别。我们的结果表明，MTA 和 MTB 形成了一个精细的多功能蛋白复合物，可以识别自我和非自我交配类型的细胞，从而分别抑制或激活交配。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2855/10901506/714a4ef1bf81/elife-93770-fig7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2855/10901506/30169e3b0116/elife-93770-fig1.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2855/10901506/5181c9bb4c69/elife-93770-fig4-figsupp1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2855/10901506/c3d009abf28e/elife-93770-fig4-figsupp2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2855/10901506/4a1a784673d2/elife-93770-fig5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2855/10901506/7d290780b4f1/elife-93770-fig5-figsupp1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2855/10901506/b5f8134ac08a/elife-93770-fig5-figsupp2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2855/10901506/dae0ab7f8a7a/elife-93770-fig6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2855/10901506/714a4ef1bf81/elife-93770-fig7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2855/10901506/30169e3b0116/elife-93770-fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2855/10901506/23ff0450acf3/elife-93770-fig1-figsupp1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2855/10901506/8e02fd058dce/elife-93770-fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2855/10901506/7657ecdbc849/elife-93770-fig2-figsupp1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2855/10901506/949aa408912d/elife-93770-fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2855/10901506/95895f039f2c/elife-93770-fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2855/10901506/5181c9bb4c69/elife-93770-fig4-figsupp1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2855/10901506/c3d009abf28e/elife-93770-fig4-figsupp2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2855/10901506/4a1a784673d2/elife-93770-fig5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2855/10901506/7d290780b4f1/elife-93770-fig5-figsupp1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2855/10901506/b5f8134ac08a/elife-93770-fig5-figsupp2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2855/10901506/dae0ab7f8a7a/elife-93770-fig6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2855/10901506/714a4ef1bf81/elife-93770-fig7.jpg

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