Augustine P C
Poult Sci. 1985 Dec;64(12):2296-9. doi: 10.3382/ps.0642296.
Exposure of primary turkey kidney cell cultures to 100 micrograms/ml wheat germ agglutinin (WGA), a lectin that binds N-acetylglucosamine (NAcGl), significantly inhibited invasion of the cells by Eimeria meleagrimitis sporozoites. However, neither succinyl-WGA, a lectin that retains an affinity for NAcGl but does not bind sialic acid, nor pokeweed mitogen, another lectin that binds NAcGl, similarly inhibited invasion. Collectively, the data suggested that the inhibition of invasion may be caused primarily by binding of WGA to anionic moieties on the host cell surface and not to specific binding to NAcGl. Exposure of cells to concanavalin A, phytohemagglutinin, or Ricinus communis lectin, as well as with pokeweed mitogen, failed to inhibit invasion by the sporozoites. Ultraviolet microscopy, using fluorescein-conjugated lectins, showed that the lectins had bound to the surface of the cultured cells. No binding of NAcGl or lectins to the surfaces of the sporozoites was demonstrated by either the clumping of the parasites in different lectin concentrations or the use of fluorescein-conjugated lectins. However, exposure of E. meleagrimitis sporozoites to NAcGl increased invasion of untreated cells by 50%.
将原代火鸡肾细胞培养物暴露于100微克/毫升的麦胚凝集素（WGA），一种能结合N-乙酰葡糖胺（NAcGl）的凝集素，可显著抑制巨型艾美耳球虫子孢子对细胞的侵袭。然而，琥珀酰-WGA，一种对NAcGl仍有亲和力但不结合唾液酸的凝集素，以及商陆丝裂原，另一种能结合NAcGl的凝集素，均未产生类似的侵袭抑制作用。总体而言，数据表明侵袭抑制可能主要是由WGA与宿主细胞表面阴离子部分的结合引起的，而非与NAcGl的特异性结合。将细胞暴露于伴刀豆球蛋白A、植物血凝素或蓖麻凝集素，以及商陆丝裂原，均未能抑制子孢子的侵袭。使用荧光素偶联凝集素的紫外线显微镜观察显示，凝集素已结合到培养细胞的表面。通过不同凝集素浓度下寄生虫的凝集或使用荧光素偶联凝集素，均未证明NAcGl或凝集素与子孢子表面有结合。然而，将巨型艾美耳球虫子孢子暴露于NAcGl可使未处理细胞的侵袭增加50%。
