Murphy G F, Fonferko E, Flotte T, Bhan A K
Arch Dermatol Res. 1985;278(2):126-32. doi: 10.1007/BF00409219.
We observed the apparent migration of Langerhans cells across the basal lamina of normal human skin by immunoelectron microscopy using monoclonal anti-T6 antibody. This technique made it possible to visualize cytoplasmic processes of Langerhans cells not normally detectable by routine transmission electron microscopy, and therefore facilitated the documentation of the migratory process. Although events early in the migratory sequence were not observed, perhaps as the result of the evanescent nature of this phase, the association of Langerhans cells with focal disruptions in the epidermal basal lamina was documented. The basal lamina adjacent to these Langerhans cells was electron lucent, granular in character, and thinned, or intact, suggesting sequential reassembly after disruption. This study provides ultrastructural documentation supporting the hypothesis of ongoing migration of Langerhans cells across epidermal membranes, and suggests that this process is mediated by the disruption and reconstitution of the epidermal basal lamina.
我们使用单克隆抗T6抗体，通过免疫电子显微镜观察了朗格汉斯细胞在正常人皮肤基底层的明显迁移。这项技术能够使朗格汉斯细胞的细胞质突起可视化，而这些突起在常规透射电子显微镜下通常无法检测到，因此有助于记录迁移过程。尽管迁移序列早期的事件未被观察到，这可能是由于该阶段短暂性的缘故，但朗格汉斯细胞与表皮基底层局灶性破坏的关联已被记录下来。与这些朗格汉斯细胞相邻的基底层电子透明、呈颗粒状且变薄或完整，提示破坏后有顺序的重新组装。本研究提供了超微结构证据，支持朗格汉斯细胞持续穿越表皮膜迁移的假说，并表明该过程是由表皮基底层的破坏和重建介导的。
