School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing, China.
Beijing Union Pharmaceutical Factory, Beijing, China.
J Ethnopharmacol. 2024 May 23;326:117981. doi: 10.1016/j.jep.2024.117981. Epub 2024 Feb 28.
Sanchen powder is a traditional Tibetan medicine comprising Bambusae Concretio Silicea, Carthami Flos, and Bovis Calculus Artifactus. Bambusae Concretio Silicea is the dried mass of secreted fluid in the stalks of Gramineae plants such as Bambusa textilis McClure or Schizostachyum chinense Rendle. Carthami Flos is the dried flower of Carthamus tinctorius L. in the Compositae plant. Bovis Calculus Artifactus is made from ox bile powder, cholic acid, hyodeoxycholic acid, taurine, bilirubin, cholesterol, and trace elements. Research has evidenced the antibacterial efficacy of Sanchen powder, albeit its active constituents for this effect are yet to be established.
To investigate effective compounds, potential targets, and molecular mechanism of Sanchen powder for its antibacterial properties by using network pharmacology combined with in vitro validation, with the aims of observing the action of effective compounds in Sanchen powder and exploring new therapeutic strategies for antibacterial.
In this study, UPLC-Q-TOF-MS was utilized to identify the chemical composition in Sanchen powder and its blood-borne chemical ingredients post-oral intake. A network pharmacology analysis was used to establish the chemical compound in the blood following oral administration-target-disease network. The study aimed to identify antibacterial active ingredients, which were then subjected to molecular docking and pharmacodynamic experiments to verify their efficacy.
The findings demonstrate that following oral administration, the blood contains seven key components of Sanchen powder, including bilirubin, glycochenodeoxycholic acid, glycocholic acid, taurocholic acid, phenylalanine, safflomin A, and tryptophan. Additionally, the network pharmacology and molecular docking study results indicate the potential antibacterial effects of bilirubin, glycocholic acid, and glycochenodeoxycholic acid. In vitro antibacterial experiments revealed that bilirubin, glycocholic acid, and glycochenodeoxycholic acid could restrict the growth of the Staphylococcus aureus cell membrane at a certain concentration. Moreover, they exhibited antibacterial effects on Staphylococcus aureus, Pseudomonas aeruginosa, Staphylococcus epidermidis, and Escherichia coli.
Bilirubin, glycocholic acid, and glycochenodeoxycholic acid could be effective therapeutic ingredients for the antibacterial effects of Sanchen powder. These results offer a foundation for further clinical application and research on the antibacterial effect of Sanchen powder, a Traditional Tibetan Medicine.
三臣散是一种传统的藏药，由矽藻土、红花和人工牛黄组成。矽藻土是禾本科植物如翠竹或中华苦竹茎秆中分泌的液体干燥而成的团块。红花是菊科植物红花的干燥花。人工牛黄是由牛胆汁粉、胆酸、去氧胆酸、牛磺酸、胆红素、胆固醇和微量元素制成的。研究已经证明了三臣散的抗菌功效，尽管其抗菌作用的活性成分尚未确定。
本研究采用网络药理学结合体外验证的方法，探讨三臣散的抗菌作用的有效化合物、潜在靶点和分子机制，旨在观察三臣散中有效化合物的作用，探索抗菌的新治疗策略。
本研究采用 UPLC-Q-TOF-MS 鉴定三臣散及其口服后血中移行成分的化学成分。采用网络药理学分析建立口服后血中化合物-靶-病网络。研究旨在筛选出抗菌活性成分，然后进行分子对接和药效学实验验证其功效。
研究结果表明，口服后，血液中含有三臣散的七种关键成分，包括胆红素、甘氨脱氧胆酸、甘胆酸、牛磺胆酸、苯丙氨酸、沙弗洛明 A 和色氨酸。此外，网络药理学和分子对接研究结果表明，胆红素、甘胆酸和甘氨脱氧胆酸具有潜在的抗菌作用。体外抗菌实验表明，胆红素、甘胆酸和甘氨脱氧胆酸可在一定浓度下限制金黄色葡萄球菌细胞膜的生长。此外，它们对金黄色葡萄球菌、铜绿假单胞菌、表皮葡萄球菌和大肠杆菌均有抗菌作用。
胆红素、甘胆酸和甘氨脱氧胆酸可能是三臣散抗菌作用的有效治疗成分。这些结果为进一步临床应用和研究三臣散作为一种传统藏药的抗菌作用提供了基础。
