Botany and Microbiology Department, Faculty of Science, Helwan University, Ain Helwan, Cairo, 11795, Egypt.
Botany and Microbiology Department, Faculty of Science, Helwan University, Ain Helwan, Cairo, 11795, Egypt.
Microb Pathog. 2024 Apr;189:106603. doi: 10.1016/j.micpath.2024.106603. Epub 2024 Feb 28.
Isolation of novel bioactive metabolites from Streptomyces strains is a promising source for drug discovery. However, conventional screening approaches have limitations in identifying new leads due to redundant discoveries. Optimization of culture conditions is important but traditionally optimized one factor at a time, failing to consider interactions. This study addressed these gaps by enhancing metabolite production from Streptomyces thinghirensis WAE1 through statistical optimization. Various chemical and physical factors impacting metabolite production were identified. Response surface methodology with a central composite design was applied to optimize significant factors like carbon source, nitrogen source, inoculum size, pH, temperature and incubation period. This optimized production against Streptococcus pneumoniae, increasing antibacterial activity by 74.92%. Gas chromatography-mass spectrometry revealed 19 bioactive compounds, including 1,25-dihydroxyvitamin D3 inhibiting cell wall development. This highlights S. thinghirensis WAE1's potential as a bioresource and emphasizes studying metabolite production from novel Streptomyces strains to discover new antibacterial drugs.
从链霉菌菌株中分离新型生物活性代谢产物是药物发现的有前途的来源。然而，由于冗余发现，传统的筛选方法在鉴定新的先导化合物方面存在局限性。优化培养条件很重要，但传统上一次优化一个因素，未能考虑相互作用。本研究通过统计优化来增强 thinghirensis WAE1 链霉菌的代谢产物生产，从而解决了这些空白。确定了影响代谢产物生产的各种化学和物理因素。应用中心组合设计的响应面法优化了碳源、氮源、接种量、pH 值、温度和培养时间等重要因素。与肺炎链球菌相比，这种优化生产提高了 74.92%的抗菌活性。气相色谱-质谱法揭示了 19 种具有生物活性的化合物，包括抑制细胞壁发育的 1,25-二羟基维生素 D3。这突出了 thinghirensis WAE1 作为生物资源的潜力，并强调了从新型链霉菌菌株中研究代谢产物生产以发现新的抗菌药物的重要性。
