OBJECTIVES

It is worthwhile to note that, some probiotics such as and isolated from dairy products have significant therapeutic effects against cancer cells. Here, we evaluated anti-proliferation and the apoptotic effects of isolated Ab.RS22 from traditional dairy products on the HeLa cervical cancer cells

MATERIALS AND METHODS

The viability of treated HeLa cells with supernatant of in 0.5, 0.75, 1, 1.5, and 2 ng/ml concentrations, and IC values were detected by tetrazolium bromide. The Ab.RS22-induced cell death by flow cytometry was confirmed through evaluation of the expression of , , , and genes by quantitative reverse transcription-polymerase chain reactions (qRT-PCR).

RESULTS

Most cytotoxicity effects of on HeLa cells were detected in 2 ng/ml at 24 hr (<0.01); also, the IC value was measured as 1.5 ng/ml. The findings of the flow cytometry assay showed that Ab.RS22 in 1.5 ng/ml concentration at 24 hr increased the percentage of both apoptosis and necrosis cells. Lactobacillus-induced cell death was verified through results of Real-time PCR; where expression of , , and genes was increased (<0.01), and also expression of gene (anti-apoptotic) was decreased (<0.05).

CONCLUSION

Our findings showed that Ab.RS22 could dose-dependently inhibit the proliferation of the HeLa cells. Its apoptotic effect was confirmed via modulating / gene expression and activation of the caspase-3 mediated apoptosis pathway. Therefore, Ab.RS22 can be considered a valuable anticancer candidate against cervical cancer progression in subsequent studies.