Yoshida S, Downey J M, Yellon D M, Miura T, Iimura O
Can J Cardiol. 1985 Sep-Oct;1(5):346-53.
The calcium antagonist, diltiazem, was examined for its ability to limit infarct size and to diminish the severity and frequency of the ventricular arrhythmias in closed chest dogs following coronary occlusion. Diltiazem was administered 100 micrograms/kg as a bolus, followed by 15 micrograms/kg/min infusion for 48 h, starting 10 min after coronary occlusion (11 dogs). The control groups (9 dogs) received an equal volume of saline. Risk area and regional myocardial blood flow was measured with radioactive microspheres 5 min after coronary embolization. Forty-eight hours after embolization, the dogs were sacrificed, the hearts sectioned into 4 mm slices and the slices were stained with triphenyl tetrazolium chloride to measure the infarct size. Risk size was determined by autoradiography of the microspheres. Infarct size, expressed as a percentage of the area at risk, was significantly lower in the diltiazem treated group than in the control group (47.4 +/- 8.9 vs. 76.1 +/- 6.4%). However, the severity and frequency of the ventricular arrhythmias for the first 24 h was not any different between two groups.
研究了钙拮抗剂地尔硫䓬限制冠状动脉闭塞后闭胸犬梗死面积以及减轻室性心律失常严重程度和发作频率的能力。地尔硫䓬以100微克/千克的剂量静脉推注，随后以15微克/千克/分钟的速度输注48小时，在冠状动脉闭塞后10分钟开始给药（11只犬）。对照组（9只犬）给予等量生理盐水。冠状动脉栓塞后5分钟，用放射性微球测量危险区和局部心肌血流。栓塞48小时后，处死犬只，将心脏切成4毫米厚的切片，切片用氯化三苯基四氮唑染色以测量梗死面积。通过微球的放射自显影确定危险区大小。梗死面积以危险区面积的百分比表示，地尔硫䓬治疗组明显低于对照组（47.4±8.9%对76.1±6.4%）。然而，两组在前24小时室性心律失常的严重程度和发作频率并无差异。
