Jurišić Vladimir
University of Kragujevac, Faculty of Medical Sciences, Kragujevac, Serbia.
Appl Radiat Isot. 2024 Apr;206:111251. doi: 10.1016/j.apradiso.2024.111251. Epub 2024 Feb 24.
NK (Natural killer) cells are a special population of peripheral blood lymphocytes that kill virus-infected cells as well as tumor cells. For testing NK cell function, the classic gold standard assay has been used for a long time, determining the activity from target tumor cells using radioactive chromium in cell cultures for 4h. In this study two hematological cell lines K562 and MDS where used and target and results showed different sensitivity to killing by NK cells separated from healthy volunteers. Results have been shown that MDS release significantly more radioactive chromium indicating higher degree of necrosis during cell culture. In addition, K562 cell line is better target for NK killing in all different E:T ratio in comparison to MDS cell line previously described. Based on this, it is suggested that K562 cells be continues used in the future as better target for investigation NK killing.
自然杀伤（NK）细胞是外周血淋巴细胞的一个特殊亚群，可杀伤病毒感染细胞和肿瘤细胞。长期以来，经典的金标准检测方法一直用于检测NK细胞功能，即在细胞培养中使用放射性铬在4小时内从靶肿瘤细胞中测定活性。在本研究中，使用了两种血液学细胞系K562和MDS，靶细胞和结果显示，从健康志愿者中分离出的NK细胞对其杀伤的敏感性不同。结果表明，MDS释放出显著更多的放射性铬，表明细胞培养过程中的坏死程度更高。此外，与先前描述的MDS细胞系相比，在所有不同的效靶比下，K562细胞系都是NK杀伤的更好靶细胞。基于此，建议未来继续使用K562细胞作为研究NK杀伤作用的更好靶细胞。
