Takizawa Yuki, Morino Tsunetaro, Takagi Ryo, Otori Nobuyoshi, Kojima Hiromi, Yamato Masayuki
Institute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University, 8-1 Kawada-cho, Shinjuku-ku, Tokyo, Japan.
Department of Otorhinolaryngology, Jikei University School of Medicine, 3-25-8 Nishi-Shimbashi, Minato-ku, Tokyo, Japan.
Regen Ther. 2024 Feb 23;25:387-394. doi: 10.1016/j.reth.2024.02.001. eCollection 2024 Mar.
The treatment of nasal septum perforation solely by surgical intervention presents significant challenges. This study evaluated the effect of basic fibroblast growth factor (bFGF) in combination with collagen/gelatin on wound healing of nasal septum perforation in a rabbit animal model.
A nasal septum perforation rabbit model was created. bFGF was added to a collagen/gelatin fixture and placed adjacent to the perforation, which is a complete defect. The rabbits were divided into three groups: the sham group that underwent the surgical procedure only, bFGF (-) group that received collagen/gelatin fixture without bFGF, and bFGF(+) group that received collagen/gelatin fixture with bFGF. The dimensions of the perforations were measured after 4 weeks, and the septum was subjected to histological examination.
All perforations remained open in the sham group (closure rate: 20.4%-83.1%). The closure rates of the bFGF(-) and bFGF(+) groups were 49.4%-68.8% and 72.7%-100%, respectively. No significant difference was noted in the closure rates between the sham and bFGF(-) groups; however, significant differences were observed between the sham and bFGF(+) groups, and the bFGF(-) and bFGF(+) groups (p < 0.05), indicating that bFGF promoted perforation closure.
The study demonstrated that bFGF with collagen/gelatin carrier promoted wound healing in a rabbit model of nasal septum perforation.
仅通过手术干预治疗鼻中隔穿孔存在重大挑战。本研究在兔动物模型中评估了碱性成纤维细胞生长因子（bFGF）与胶原蛋白/明胶联合应用对鼻中隔穿孔伤口愈合的影响。
建立鼻中隔穿孔兔模型。将bFGF添加到胶原蛋白/明胶固定装置中，并放置在穿孔（完全缺损）附近。将兔子分为三组：仅接受手术操作的假手术组、接受不含bFGF的胶原蛋白/明胶固定装置的bFGF（-）组和接受含bFGF的胶原蛋白/明胶固定装置的bFGF（+）组。4周后测量穿孔尺寸，并对鼻中隔进行组织学检查。
假手术组所有穿孔均未闭合（闭合率：20.4%-83.1%）。bFGF（-）组和bFGF（+）组的闭合率分别为49.4%-68.8%和72.7%-100%。假手术组与bFGF（-）组之间的闭合率无显著差异；然而，假手术组与bFGF（+）组之间以及bFGF（-）组与bFGF（+）组之间观察到显著差异（p<0.05），表明bFGF促进了穿孔闭合。
该研究表明，bFGF与胶原蛋白/明胶载体可促进兔鼻中隔穿孔模型的伤口愈合。
