Wang Shanshan, Liu Shima, Cao Shuting, Bao Yunhui, Wang Lihua, He Zhengliang Eric, Li Jiang, Zhou Yi, Lv Min
College of Chemistry and Materials Science, Shanghai Normal University, Shanghai 200234, China.
Key Laboratory of Hunan Forest Products and Chemical Industry Engineering, National and Local United Engineering Laboratory of Integrative Utilization of Eucommia ulmoides, College of Chemistry and Chemical Engineering, Jishou University, Jiajie Zhang,Hunan 427000, China.
JACS Au. 2024 Feb 16;4(2):855-864. doi: 10.1021/jacsau.4c00008. eCollection 2024 Feb 26.
Graphene-based composites have shown significant potential in the treatment of biofilm infections in clinical settings due to their exceptional antimicrobial properties and specific mechanisms. Nevertheless, a comprehensive understanding of the influence exerted by nanoparticles embedded in the composites on the development and structure of biofilms is still lacking. Here, we fabricate different graphene oxide-silver nanoparticle (GAg) composite-modified substrates (GAgS) with varying densities of silver nanoparticles (AgNPs) and investigate their effects on planktonic bacterial adhesion, subsequent biofilm formation, and mature biofilm structure. Our findings indicate that the initial attachment of cells during biofilm formation is determined by the density of AgNPs on the GAgS surface. In contrast, the subsequent transition from adherent bacteria to the biofilm is determined by GAgS's synergistic antimicrobial effect. There exists a threshold for the inhibitory performance of GAgS, where the 20 μg/cm GAg composite completely prevents biofilm formation; below this concentration, GAgS delays the development of the biofilm and causes structural changes in the mature biofilm with enhanced bacterial growth and increased production of extracellular polymeric substance. More importantly, GAgS have minimal impact on mammalian cell morphology and proliferation while not inducing hemolysis in red blood cells. These results suggest that GAg composites hold promise as a therapeutic approach for addressing medical devices and implant-associated biofilm infections.
基于石墨烯的复合材料因其卓越的抗菌性能和特定机制，在临床环境中治疗生物膜感染方面已显示出巨大潜力。然而，对于复合材料中嵌入的纳米颗粒对生物膜发育和结构的影响仍缺乏全面了解。在此，我们制备了具有不同银纳米颗粒（AgNPs）密度的不同氧化石墨烯-银纳米颗粒（GAg）复合改性基质（GAgS），并研究它们对浮游细菌粘附、随后的生物膜形成以及成熟生物膜结构的影响。我们的研究结果表明，生物膜形成过程中细胞的初始附着由GAgS表面AgNPs的密度决定。相比之下，随后从粘附细菌向生物膜的转变由GAgS的协同抗菌作用决定。GAgS的抑制性能存在一个阈值，其中20μg/cm的GAg复合材料完全阻止生物膜形成；低于此浓度，GAgS会延迟生物膜的发育，并导致成熟生物膜的结构变化，伴有细菌生长增强和细胞外聚合物产量增加。更重要的是，GAgS对哺乳动物细胞形态和增殖的影响极小，同时不会诱导红细胞溶血。这些结果表明，GAg复合材料有望成为解决医疗器械和植入相关生物膜感染的一种治疗方法。
