Laboratory for Stem Cell & Restorative Neurology, Department of Biotechnology, Era's Lucknow Medical College and Hospital, Era University, Lucknow, India.
Department of Stem Cell Biology and Regenerative Medicine, Era's Lucknow Medical College Hospital, Era University, Lucknow, India.
Methods Mol Biol. 2024;2761:623-633. doi: 10.1007/978-1-0716-3662-6_41.
Stroke is the third-leading cause of death and the leading cause of acquired adult disability worldwide. Several ischemic stroke models are currently available. However, mimicking focal cerebral ischemia (FCI) is the most common. The formation of an embolic or thrombotic occlusion at or near the middle cerebral artery causes most events in FCI. The current protocol closely mimics the etiology of human stroke and ensures that the results obtained are highly relevant. The method described in this protocol yields reproducible results. The success of this model in ischemic research can be examined through the utilization of Doppler blood flow imaging equipment.
中风是全球范围内的第三大致死原因和导致成人后天残疾的首要原因。目前有几种缺血性中风模型，但模拟局灶性脑缺血（FCI）最为常见。大脑中动脉或其附近的栓塞或血栓形成导致大多数 FCI 事件的发生。目前的方案紧密模拟了人类中风的病因，确保了所获得的结果具有高度相关性。本方案中描述的方法可产生可重复的结果。通过利用多普勒血流成像设备，可以检验该模型在缺血性研究中的成功。
