Embrapa Recursos Genéticos e Biotecnologia, PqEB Parque de Estação Biológica, Brasília, DF, Brazil.
Scientia Terrae Research Institute, Sint-Katelijne-Waver, Belgium.
Methods Mol Biol. 2024;2756:227-245. doi: 10.1007/978-1-0716-3638-1_5.
Among plant-parasitic nematodes, root-knot nematodes (RKN), Meloidogyne spp., are the most important parasite infecting economically important crops globally and causing severe losses in crop production. The use of efficient nematode control methods against these parasites depends upon their correct detection in roots and soil samples. Currently, the use of integrated identification methods, including biochemical, molecular, and morphological-based characters, is preferred. But the techniques using morphology and phylogenetic analysis are time-consuming and not suitable for routine analysis. They have only been used for studies of cryptic species, which were identified using integrative taxonomy. Here we describe the enzymatic and molecular-based methods that have successfully been used in Brazil for more than 25 years in the Nematology Lab at Embrapa Genetic Resources and Biotechnology for routine analysis. This technique is a combination of isozyme esterase profiling and molecular markers, with the aim of having a rapid and correct diagnosis of Meloidogyne spp. populations from field and greenhouse.
在植物寄生线虫中，根结线虫（RKN），尤其是根结线虫属（Meloidogyne spp.），是全球范围内感染重要经济作物的最重要寄生虫，对作物生产造成严重损失。针对这些寄生虫的高效线虫防治方法的应用取决于在根和土壤样本中对它们的正确检测。目前，包括生化、分子和形态学特征在内的综合鉴定方法的应用更为普遍。但是，基于形态学和系统发育分析的技术耗时且不适合常规分析。它们仅用于隐种的研究，这些隐种是通过综合分类学方法来鉴定的。在这里，我们描述了在巴西已经成功使用了 25 年以上的基于酶和分子的方法，这些方法在 Embrapa 遗传资源与生物技术的线虫学实验室中用于常规分析。该技术是同工酶酯酶图谱和分子标记的结合，旨在实现田间和温室中根结线虫属种群的快速和正确诊断。
