Borriss R, Hofemeister J
Mol Gen Mikrobiol Virusol. 1985 Feb(2):21-6.
Beta-glucanase gene from Bacillus subtilis 168 has been mapped by bacteriophage pBS1 transduction technique between sacA and purA genes. The stimulating effect of pleiotropic mutations pap, amyB and sacUh on beta-glucanase production in Bacillus subtilis and Bacillus amyloliquefaciens has been described. Beta-glucanase gene from Bacillus amyloliquefaciens has been cloned ona Charon 4A vector. Expression of the gene in E. coli cells depended on the orientation of the cloned DNA on a pBR322 vector plasmid. Maximal enzymatic activity was registered in periplasm. Beta-glucanase gene was recloned in Bacillus subtilis cells. Bacillus subtilis strain, harbouring pBG1, produces 500 times more beta-glucanase as compared with the wild type strain of Bacillus subtilis.
已通过噬菌体pBS1转导技术将枯草芽孢杆菌168的β-葡聚糖酶基因定位在sacA和purA基因之间。已描述了多效性突变pap、amyB和sacUh对枯草芽孢杆菌和淀粉液化芽孢杆菌中β-葡聚糖酶产生的刺激作用。淀粉液化芽孢杆菌的β-葡聚糖酶基因已克隆到Charon 4A载体上。该基因在大肠杆菌细胞中的表达取决于克隆DNA在pBR322载体质粒上的方向。最大酶活性出现在周质中。β-葡聚糖酶基因在枯草芽孢杆菌细胞中重新克隆。携带pBG1的枯草芽孢杆菌菌株产生的β-葡聚糖酶比枯草芽孢杆菌野生型菌株多500倍。
