Department of Bionanosystem Engineering, Graduate School, Jeonbuk National University, Jeonju 561-756, the Republic of Korea; Department of Bionanotechnology and Bioconvergence Engineering, Graduate School, Jeonbuk National University, Jeonju 561-756, the Republic of Korea.
Division of Mechanical Design Engineering, Jeonbuk National University, Jeonju 561-756, the Republic of Korea.
Colloids Surf B Biointerfaces. 2024 Apr;236:113804. doi: 10.1016/j.colsurfb.2024.113804. Epub 2024 Feb 17.
In this study, a double network (DN) hydrogel was synthesized using poly(ethylene glycol) diacrylate (PEGDA) and sodium alginate (SA), incorporating copper-doped mesoporous silica nanospheres (Cu-MSNs) and zinc oxide nanoparticles (ZnO NPs). The blending of PEGDA and SA (PS) facilitates the double network and improves the less porous microstructure of pure PEGDA hydrogel. Furthermore, the incorporation of ZnO NPs and Cu-MSNs into the hydrogel network (PS@ZnO/Cu-MSNs) improved the mechanical properties of the hydrogel (Compressive strength = ⁓153 kPa and Young's modulus = ⁓ 1.66 kPa) when compared to PS hydrogel alone (Compressive strength = ⁓ 103 kPa and Young's modulus = ⁓ 0.95 kPa). In addition, the PS@ZnO/Cu-MSNs composite hydrogel showed antibacterial activities against Staphylococcus aureus and Escherichia coli. Importantly, the PS@ZnO/Cu-MSNs hydrogel demonstrated excellent biocompatibility, enhanced MC3T3-E1 cell adhesion, proliferation, and significant early-stage osteoblastic differentiation, as evidenced by increased alkaline phosphatase (ALP), and improved calcium mineralization, as evidenced by increased alizarin red staining (ARS) activities. These findings point to the possible use of the PS@ZnO/Cu-MSNs composite hydrogel in bone tissue regeneration.
在这项研究中，使用聚乙二醇二丙烯酸酯（PEGDA）和海藻酸钠（SA）合成了一种双网络（DN）水凝胶，其中掺入了铜掺杂介孔硅纳米球（Cu-MSNs）和氧化锌纳米粒子（ZnO NPs）。PEGDA 和 SA 的混合（PS）促进了双网络的形成，并改善了纯 PEGDA 水凝胶的较少多孔微观结构。此外，将 ZnO NPs 和 Cu-MSNs 掺入水凝胶网络（PS@ZnO/Cu-MSNs）中，与单独的 PS 水凝胶（压缩强度= ⁓103 kPa 和杨氏模量= ⁓0.95 kPa）相比，提高了水凝胶的机械性能（压缩强度= ⁓153 kPa 和杨氏模量= ⁓1.66 kPa）。此外，PS@ZnO/Cu-MSNs 复合水凝胶对金黄色葡萄球菌和大肠杆菌表现出抗菌活性。重要的是，PS@ZnO/Cu-MSNs 水凝胶表现出优异的生物相容性，增强了 MC3T3-E1 细胞的黏附、增殖能力，并在早期阶段显著促进成骨分化，碱性磷酸酶（ALP）活性增加，茜素红染色（ARS）活性增强表明钙矿化得到改善。这些发现表明，PS@ZnO/Cu-MSNs 复合水凝胶可能用于骨组织再生。
