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一种基于异佛尔酮的新型近红外荧光和比色探针用于 Al 感应及其在活细胞和植物成像中的应用。

A novel isophorone-based NIR fluorescent and colormetric probe for Al sensing and its application for living cells and plants imaging.

机构信息

Department of Applied Chemistry, College of Science, Northeast Agricultural University, Harbin 150030, PR China.

Department of Applied Chemistry, College of Science, Northeast Agricultural University, Harbin 150030, PR China.

出版信息

Spectrochim Acta A Mol Biomol Spectrosc. 2024 May 5;312:124040. doi: 10.1016/j.saa.2024.124040. Epub 2024 Feb 19.

Abstract

In this paper, an isophorone-based NIR fluorescent and colormetric probe BDDH for Al was synthesized and characterized, it showed highly selectivity and sensitivity through significant fluorescence enhancement and visible color change towards Al. The job plot confirmed that the binding ratio of BDDH with Al was 1:1. Furthermore, the limit of detection (LOD) of Al was determined to be 4.01 × 10 M. Moreover, BDDH was successfully applicated in identification of Al in the different water samples, cell imaging in alive MCF-7 cells and plant imaging in soybean roots.

摘要

本文合成并表征了一种基于异佛尔酮的近红外荧光和比色探针 BDDH,用于检测 Al。它对 Al 表现出高度的选择性和灵敏度,通过显著的荧光增强和可见颜色变化来检测 Al。Job 图证实 BDDH 与 Al 的结合比为 1:1。此外,Al 的检测限(LOD)被确定为 4.01×10-7 M。此外,BDDH 成功应用于不同水样中 Al 的识别、活 MCF-7 细胞中的细胞成像和大豆根中的植物成像。

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