College of Chemistry and Materials Engineering, Hunan University of Arts and Science, Hunan Provincial Key Laboratory of Water Treatment Functional Materials, Changde 415000, PR China.
College of Chemistry and Materials Engineering, Hunan University of Arts and Science, Hunan Provincial Key Laboratory of Water Treatment Functional Materials, Changde 415000, PR China.
Spectrochim Acta A Mol Biomol Spectrosc. 2024 May 5;312:124064. doi: 10.1016/j.saa.2024.124064. Epub 2024 Feb 24.
As two of important highly reactive species / nitrogen species, hypochloric acid (HClO) and peroxynitrite (ONOO) are involved in various pathological and physiological processes, which are important factors that affect and reflect the functional state of lysosome. Nevertheless, many of their roles are still indefinite because of lack of suitable analytical methods for HClO and ONOO detection in lysosome. Herein, we designed a lysosome-targeted probe to monitor HClO and ONOO, which was a hydrid of the benzothiazole derivative, methyl thioether (HClO recognition site) and morpholino hydrazone (ONOO recognition and lysosome target site). The probe exhibited high sensitivity, good selectivity and fast response toward HClO and ONOO without spectral crosstalk, and can be employed for quantitative monitoring HClO and ONOO with LOD of 63 and 83 nM, respectively. In addition, the dual-site probe was lysosome targetable and could be used for detection of HClO and ONOO in living cells. Furthermore, the excellent behavior made it was suitable for imaging of HClO and ONOO in zebrafish. Thus, the present probe provides a potent tool for distinguishing monitoring HClO and ONOO and exploring the role of HClO and ONOO in biological systems.
作为两种重要的高反应性物质/氮物质，次氯酸 (HClO) 和过氧亚硝酸盐 (ONOO) 参与各种病理和生理过程，是影响和反映溶酶体功能状态的重要因素。然而，由于缺乏用于检测溶酶体中 HClO 和 ONOO 的合适分析方法，它们的许多作用仍然不确定。在此，我们设计了一种用于监测 HClO 和 ONOO 的溶酶体靶向探针，它是苯并噻唑衍生物、甲基硫醚 (HClO 识别位点) 和吗啉腙 (ONOO 识别和溶酶体靶位点) 的混合物。该探针对 HClO 和 ONOO 具有高灵敏度、良好的选择性和快速响应，没有光谱串扰，并且可以用于定量监测 HClO 和 ONOO，LOD 分别为 63 和 83 nM。此外，该双位点探针可靶向溶酶体，并可用于检测活细胞中的 HClO 和 ONOO。此外，优异的性能使其适用于斑马鱼中 HClO 和 ONOO 的成像。因此，本探针为区分监测 HClO 和 ONOO 以及探索 HClO 和 ONOO 在生物系统中的作用提供了有力的工具。
