Department of Chemistry, University of Nebraska-Lincoln, Lincoln, Nebraska 68588, United States.
Department of Chemistry, University of Nebraska-Lincoln, Lincoln, Nebraska 68588, United States; The Nebraska Center for Integrated Biomolecular Communication (NCIBC), University of Nebraska-Lincoln, Lincoln, Nebraska 68588, United States.
J Biotechnol. 2024 Apr 10;385:1-12. doi: 10.1016/j.jbiotec.2024.02.013. Epub 2024 Feb 28.
Emerging consumer demand for safer, more sustainable flavors and fragrances has created new challenges for the industry. Enzymatic syntheses represent a promising green production route, but the broad application requires engineering advancements for expanded diversity, improved selectivity, and enhanced stability to be cost-competitive with current methods. This review discusses recent advances and future outlooks for enzyme engineering in this field. We focus on carboxylic acid reductases (CARs) and unspecific peroxygenases (UPOs) that enable selective productions of complex flavor and fragrance molecules. Both enzyme types consist of natural variants with attractive characteristics for biocatalytic applications. Applying protein engineering methods, including rational design and directed evolution in concert with computational modeling, present excellent examples for property improvements to unleash the full potential of enzymes in the biosynthesis of value-added chemicals.
新兴的消费者对更安全、更可持续的香精和香料的需求给行业带来了新的挑战。酶合成代表了一种有前途的绿色生产途径，但广泛应用需要工程学的进步，以扩大多样性、提高选择性和增强稳定性，从而在成本上具有竞争力，超越当前的方法。本文综述了该领域酶工程的最新进展和未来展望。我们专注于羧酸还原酶（CARs）和非特异性过氧化物酶（UPOs），它们能够选择性地生产复杂的香精和香料分子。这两种酶都由具有吸引力的生物催化应用特性的天然变体组成。应用蛋白质工程方法，包括理性设计和定向进化，以及计算建模，为提高性能提供了极好的例子，从而充分发挥酶在增值化学品生物合成中的潜力。
