Department of Biocatalysis, Institute of Catalysis, CSIC, Marie Curie 2, 28049 Cantoblanco, Madrid, Spain.
EvoEnzyme S.L., Parque Científico de Madrid, 28049, Madrid. Electronic address: https://twitter.com/@evoenzyme.
Curr Opin Struct Biol. 2022 Apr;73:102342. doi: 10.1016/j.sbi.2022.102342. Epub 2022 Feb 28.
The selective insertion of oxygen into non-activated organic molecules has to date been considered of utmost importance to synthesize existing and next generation industrial chemicals or pharmaceuticals. In this respect, the minimal requirements and high activity of fungal unspecific peroxygenases (UPOs) situate them as the jewel in the crown of C-H oxyfunctionalization biocatalysts. Although their limited availability and development has hindered their incorporation into industry, the conjunction of directed evolution and computational design is approaching UPOs to practical applications. In this review, we will address the most recent advances in UPO engineering, both of the long and short UPO families, while discussing the future prospects in this fast-moving field of research.
迄今为止，选择性地将氧插入非活化的有机分子被认为是合成现有和下一代工业化学品或药物的最重要手段。在这方面，真菌非特异性过氧化物酶（UPOs）的最小要求和高活性使它们成为 C-H 氧化官能化生物催化剂中的瑰宝。尽管它们的有限可用性和发展阻碍了它们在工业中的应用，但定向进化和计算设计的结合正在使 UPO 接近实际应用。在这篇综述中，我们将讨论长、短 UPO 家族的 UPO 工程的最新进展，同时讨论这个快速发展的研究领域的未来前景。
