Department of Chemical and Food Engingeheering, Federal University of Santa Catarina, Florianópolis, SC, Brazil.
Department of Chemical and Food Engingeheering, Federal University of Santa Catarina, Florianópolis, SC, Brazil; Department of Textile Engineering, Federal University of Santa Catarina, Blumenau, SC, Brazil.
Int J Biol Macromol. 2024 Apr;263(Pt 1):130513. doi: 10.1016/j.ijbiomac.2024.130513. Epub 2024 Feb 28.
Anthocyanins (ACNs) are natural compounds with potential applications due to their colorimetric response to pH. Due to their sensitivity to various environmental factors, nanoencapsulation with biopolymers is a successful strategy for stabilizing ACNs. In this work ACNs were extracted from grape skins and encapsulated into chitosan (CS) nanoparticles by ionic gelation using sodium tripolyphosphate (TPP) as a cross-linking agent. CS nanoparticles loaded with ACNs had particle sizes between 291 and 324 nm and polydispersity index around 0.3. The encapsulation efficiency of ACNs was approximately 60 %; and encapsulated anthocyanins (ACN-NPs) exhibited color change properties under different pH conditions. pH-sensitive labels based on polyvinyl alcohol (PVA) were prepared by the casting method. The effect of incorporating ACN-NPs on the physical, structural, and pH-sensitive properties of PVA labels was evaluated, and its application as shrimp freshness indicator was studied. The nanoencapsulation protected ACNs against heat and light treatments, preserving the original purple color. When applying the label, visible changes from red to blue until reaching yellow were observed with the change in the quality of the shrimp at the refrigeration temperature. The results suggest that PVA labels containing ACNs encapsulated in C-NPs can be used as smart packaging labels in the food industry.
花色苷（ACNs）是一类具有颜色响应 pH 值特性的天然化合物，具有潜在的应用价值。由于其对各种环境因素敏感，因此，采用生物聚合物进行纳米封装是稳定 ACNs 的一种成功策略。在这项工作中，从葡萄皮中提取花色苷，并通过离子凝胶化作用，使用三聚磷酸钠（TPP）作为交联剂，将其封装到壳聚糖（CS）纳米颗粒中。负载花色苷的 CS 纳米颗粒的粒径在 291 至 324nm 之间，多分散指数约为 0.3。花色苷的包封效率约为 60%；并且封装的花色苷（ACN-NPs）在不同 pH 条件下表现出色相变化特性。通过浇铸法制备了基于聚乙烯醇（PVA）的 pH 敏感标签。评估了将 ACN-NPs 掺入 PVA 标签对其物理、结构和 pH 敏感性质的影响，并研究了其作为虾类新鲜度指示剂的应用。纳米封装保护 ACNs 免受热和光处理的影响，保持了原始的紫色。当应用标签时，在冷藏温度下，随着虾的质量变化，可以观察到从红色到蓝色再到黄色的可见变化。结果表明，含有封装在 CS-NPs 中的 ACN 的 PVA 标签可用作食品工业中的智能包装标签。
