Indian Council of Agricultural Research - Central Institute of Fisheries Education, Versova, Mumbai, Maharashtra 400061, India. Electronic address: https://twitter.com/pintonevil8.
Department of Biosciences and Bioengineering, Indian Institute of Technology Bombay, Powai, Mumbai 400076, India.
Comp Biochem Physiol Part D Genomics Proteomics. 2024 Jun;50:101221. doi: 10.1016/j.cbd.2024.101221. Epub 2024 Feb 26.
Edwardsiella tarda (Et) is a zoonotic gram-negative pathogen with a diverse host range, including fish. However, the in-depth molecular mechanisms underlying the response of Labeo rohita (rohu) kidney to Et are poorly understood. A proteomic and histopathological analysis was performed for the rohu kidney after Et infection. The histopathology of the infected rohu kidney showed vacuolation and necrosis. After LC-MS/MS analysis, ~1240 proteins were identified with ≥2 unique peptides. A total of 96 differentially abundant proteins (DAPs) were observed between the control and Et infected group (ET). Metascape and STRING analysis were used for the gene ontology (GO), and protein-protein interaction network (PPI) for the significant pathways of DAPs. In PPI, low-abundant proteins were mapped to metabolic pathways and oxidative phosphorylation (cox5ab, uqcrfs1). High-abundance proteins were mapped to ribosomes (rplp2), protein process in the ER (hspa8), and immune system (ptgdsb.1, muc2). Our label-free proteomic approach in the rohu kidney revealed abundant enriched proteins involved in vesicle coat (ehd4), complement activation (c3a.1, c9, c7a), phagosome (thbs4, mapk1), metabolic reprogramming (hao1, glud1a), wound healing (vim, alox5), and the immune system (psap) after Et infection. A targeted proteomics approach of multiple reaction monitoring (MRM) validated the DAPs (nprl3, ambp, vmo1a, hspg2, muc2, hao1 and glud1a) between control and ET. Overall, the current analysis of histology and proteome in the rohu kidney provides comprehensive data on pathogenicity and the potential immune proteins against Et.
迟缓爱德华氏菌（Edwardsiella tarda，Et）是一种具有多种宿主范围的人畜共患革兰氏阴性病原体，包括鱼类。然而，罗非鱼肾脏对 Et 反应的深入分子机制尚不清楚。对 Et 感染后的罗非鱼肾脏进行了蛋白质组学和组织病理学分析。感染罗非鱼肾脏的组织病理学显示有空泡和坏死。经过 LC-MS/MS 分析，共鉴定出~1240 种具有≥2 个独特肽段的蛋白质。在对照组和 Et 感染组之间观察到 96 种差异丰度蛋白（DAP）。Metascape 和 STRING 分析用于基因本体（GO），以及 DAPs 的显著途径的蛋白质-蛋白质相互作用网络（PPI）。在 PPI 中，低丰度蛋白质映射到代谢途径和氧化磷酸化（cox5ab，uqcrfs1）。高丰度蛋白质映射到核糖体（rplp2）、内质网中的蛋白质加工（hspa8）和免疫系统（ptgdsb.1，muc2）。我们在罗非鱼肾脏中使用无标记蛋白质组学方法，揭示了大量与囊泡外壳（ehd4）、补体激活（c3a.1、c9、c7a）、吞噬体（thbs4、mapk1）、代谢重编程（hao1、glud1a）、伤口愈合（vim、alox5）和免疫系统（psap）相关的丰富富集蛋白。靶向蛋白质组学多重反应监测（MRM）方法验证了对照组和 ET 之间的 DAP（nprl3、ambp、vmo1a、hspg2、muc2、hao1 和 glud1a）。总的来说，目前对罗非鱼肾脏的组织学和蛋白质组学分析提供了关于致病性和针对 Et 的潜在免疫蛋白的全面数据。
