Department of Otolaryngology-Head and Neck Surgery, Xijing Hospital, Air Force Medical University, Xi'an, 710032, China.
Department of Otolaryngology-Head and Neck Surgery, Xijing Hospital, Air Force Medical University, Xi'an, 710032, China.
Gene Expr Patterns. 2024 Mar;51:119356. doi: 10.1016/j.gep.2024.119356. Epub 2024 Mar 2.
It can be observed from aminoglycoside-induced hair cell damage that the cochlea basal turn is more susceptible to trauma than the apex. Drug-induced hearing loss is closely related to oxidative damage. The basilar membrane directly exposed to these ototoxic drugs exhibits differences in damage, indicating that there is an inherent difference in the sensitivity to oxidative damage from the apex to the base of the cochlea. It has been reported that the morphology and characteristics of the cochlea vary from the apex to the base. Therefore, we investigated oxidative stress-related gene expression profiles in the apical, middle, and basal turns of the cochlea. The Oxidative Stress RT Profiler™ PCR Array revealed that three of the 84 genes (Mb, Mpo, and Ncf1) were upregulated in the middle turn compared to their level in the apical turn. Moreover, eight genes (Mb, Duox1, Ncf1, Ngb, Fmo2, Gpx3, Mpo, and Gstk1) were upregulated in the basal turn compared to their level in the apical turn. The qPCR verification data were similar to that of the PCR Array. We found that MPO was expressed in the rat cochlea and protected against gentamicin-induced hair cell death. This study summarized the data for the gradient of expression of oxidative stress-related genes in the cochlea and found potential candidate targets for prevention of ototoxic deafness, which may provide new insights for cochlear pathology.
从氨基糖苷类诱导的毛细胞损伤可以看出，耳蜗底转比顶端更容易受到创伤。药物诱导的听力损失与氧化损伤密切相关。基底膜直接暴露于这些耳毒性药物，其损伤存在差异，这表明耳蜗顶端到基部对氧化损伤的敏感性存在固有差异。据报道，耳蜗的形态和特征从顶端到基部逐渐变化。因此，我们研究了耳蜗顶端、中部和底部的氧化应激相关基因表达谱。氧化应激 RT 分析 PCR 阵列显示，与顶端相比，中部有 3 个 84 个基因（Mb、Mpo 和 Ncf1）上调。此外，与顶端相比，底部有 8 个基因（Mb、Duox1、Ncf1、Ngb、Fmo2、Gpx3、Mpo 和 Gstk1）上调。qPCR 验证数据与 PCR 阵列相似。我们发现 MPO 在大鼠耳蜗中表达，并能抵抗庆大霉素诱导的毛细胞死亡。本研究总结了耳蜗中氧化应激相关基因表达梯度的数据，发现了预防耳毒性聋的潜在候选靶点，这可能为耳蜗病理学提供新的见解。
