Jiangxi Provincial Engineering Research Center for Seed-breeding and Utilization of Camphor Trees of Nanchang Institute of Technology, Nanchang Institute of Technology, Nanchang 330099, Jiangxi, China.
The High School Attached to Jiangxi Normal University, Nanchang 330038, Jiangxi, China.
Plant Dis. 2024 Jul;108(7):1976-1981. doi: 10.1094/PDIS-06-23-1169-SC. Epub 2024 Jun 27.
Anthracnose, caused by spp., is a common disease of . In this study, a strain, GZY63, was isolated from fruit of the anthracnose-resistant cultivar of "Ganzhouyou7." Plate confrontation assays and field experiments demonstrated the strong inhibitory effect of GZY63 on anthracnose, and this strain exhibited broad-spectrum resistance to nine pathogenic spp. This strain shows potential as a fungicide alternative, but genetic information on this strain is critical for its optimal use. Combining Illumina and Nanopore sequencing, we assembled a high-quality circular genome of GZY63 that contained no plasmids. The GZY63 complete genome was approximately 3.93 Mb and had an average guanine-cytosine content of 46.5%. The genome comprised 4,024 predicted coding sequences and 12 types of gene clusters involved in secondary metabolite production. This genome information provides insights into the mechanism underlying the antagonistic impact of the GZY63 strain on anthracnose and its symbiotic relationship with .
炭疽病是由 spp.引起的，是一种常见的病害。本研究从炭疽病抗性品种“赣州油 7 号”果实中分离到一株 菌株 GZY63，平板对峙试验和田间试验表明，GZY63 对炭疽病具有很强的抑制作用，对 9 种病原菌表现出广谱抗性。该菌株具有替代杀菌剂的潜力，但该菌株的遗传信息对于其最佳使用至关重要。我们结合 Illumina 和 Nanopore 测序，组装了 GZY63 的高质量环状基因组，其中不含质粒。GZY63 的全基因组约为 3.93Mb，平均鸟嘌呤-胞嘧啶含量为 46.5%。基因组包含 4024 个预测编码序列和 12 种参与次生代谢产物合成的基因簇。该基因组信息为 GZY63 菌株对炭疽病的拮抗作用及其与 共生关系的机制提供了深入了解。
