Schubert Andrea, Griesmüller Carolin, Gersdorff Nikolaus, Bürgers Ralf, Wiechens Bernhard, Wassmann Torsten
Department of Prosthodontics, University Medical Center Goettingen, Goettingen, Germany.
Department of Orthodontics, University Medical Center Goettingen, Goettingen, Germany.
Clin Exp Dent Res. 2024 Apr;10(2):e864. doi: 10.1002/cre2.864.
Magnetron sputtering was evaluated to equip surfaces of orthodontic elastomeric ligatures with silver and bismuth nanofilms.
Antibacterial properties were evaluated by the adhesion of Streptococcus mutans. Polyurethane-based elastomeric ligatures were coated with silver and bismuth nanofilms via direct current magnetron sputtering. Surface roughness (R ) and surface-free energy (SFE) were assessed. Coated specimens were incubated with S. mutans for 2 h. Adhering bacteria were visualized by Hoechst staining and quantified by an ATP-based luminescence assay. One-way analysis of variance with Tukey post hoc testing and Pearson correlation analysis were performed (p < .05) to relate bacterial adhesion to surface roughness and surface-free energy.
Elastomeric ligatures were successfully coated with silver and bismuth nanofilms. R was significantly reduced by silver coating. Silver and bismuth coatings showed significantly higher SFE than controls. Adhesion of S. mutans was significantly decreased by silver coating. No correlation between bacterial adhesion and SFE was found. Correlation between bacterial adhesion and R was positive but not statistically significant.
Magnetron sputtering proved to be a feasible method to equip orthodontic elastomeric ligatures with silver and bismuth nanofilms. Silver coatings of elastomeric ligatures may reduce white spots and carious lesions in orthodontic patients. Future research is required to stabilize coatings.
评估磁控溅射法在正畸弹性结扎丝表面制备银和铋纳米薄膜的效果。
通过变形链球菌的黏附来评估抗菌性能。采用直流磁控溅射法在聚氨酯基弹性结扎丝表面涂覆银和铋纳米薄膜。评估表面粗糙度（R）和表面自由能（SFE）。将涂覆后的样本与变形链球菌孵育2小时。通过Hoechst染色观察黏附的细菌，并通过基于ATP的发光测定法定量。进行单因素方差分析及Tukey事后检验和Pearson相关性分析（p < 0.05），以研究细菌黏附与表面粗糙度和表面自由能之间的关系。
弹性结扎丝成功涂覆了银和铋纳米薄膜。银涂层显著降低了表面粗糙度。银和铋涂层的表面自由能显著高于对照组。银涂层显著降低了变形链球菌的黏附。未发现细菌黏附与表面自由能之间存在相关性。细菌黏附与表面粗糙度之间呈正相关，但无统计学意义。
磁控溅射法被证明是一种在正畸弹性结扎丝表面制备银和铋纳米薄膜的可行方法。弹性结扎丝的银涂层可能会减少正畸患者的白斑和龋损。未来需要进一步研究以稳定涂层。
