Liu Xiuju, Gan Kang, Liu Hong, Song Xiaoqing, Chen Tianjie, Liu Chenchen
Department of General Dentistry, School and Hospital of Stomatology, Jilin University,1500 Qing Hua Road, Changchun 130021, PR China.
Dental Center, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450000, PR China.
Dent Mater. 2017 Sep;33(9):e348-e360. doi: 10.1016/j.dental.2017.06.014. Epub 2017 Jul 19.
We aimed to investigate the cytotoxicity and antibacterial properties of nano-silver-coated polyetheretherketone (PEEK) produced through magnetron sputtering and provide a theoretical basis for its use in clinical applications.
The surfaces of PEEKs were coated with nano-silver at varying thicknesses (3, 6, 9, and 12nm) through magnetron sputtering technology. The resulting coated PEEK samples were classified into the following groups according to the thickness of the nano-silver coating: PEEK-3 (3nm), PEEK-6 (6nm), PEEK-9 (9nm), PEEK-12 (12nm), and PEEK control group. The surface microstructure and composition of each sample were observed by scanning electron microscopy (SEM), atomic force microscopy (AFM), and energy dispersive spectrum (EDS) analysis. The water contact angle of each sample was then measured by contact angle meters. A cell counting kit (CCK-8) was used to analyze the cytotoxicity of the mouse fibroblast cells (L929) in the coated groups (n=5) and group test samples (n=6), negative control (polyethylene, PE) (n=6), and positive control group (phenol) (n=6). The antibacterial properties of the samples were tested by co-culturing Streptococcus mutans and Straphylococcus aureus. The bacteria that adhered to the surface of samples were observed by SEM. The antibacterial adhesion ability of each sample was then evaluated.
SEM and AFM analysis results showed that the surfaces of control group samples were smooth but compact. Homogeneous silver nano-particles (AgNPs) and nano-silver coating were uniformly distributed on the surface of the coated group samples. Compared with the control samples, the nano-silver coated samples had a significant increase in surface roughness (P<0.05) as the thickness of their nano-silver coating increased. EDS analysis showed that not only C and O but also Ag were present on the surface of the coated samples. Moreover, the water contact angle of modified samples significantly increased after nano-silver coating modification (P<0.01). CCK-8 cytotoxicity test results showed that coated samples did not exhibit cytotoxicity. The antibacterial experimental results showed that the nano-silver coating can significantly improve the antibacterial activity and bacterial adhesion ability of the PEEK samples.
The compact and homogeneous nano-silver coating was successfully prepared on the surface of PEEK through magnetron sputtering. The nano-silver coated PEEKs demonstrated enhanced antibacterial activities and bacterial adhesion abilities and had no cytotoxic effects.
我们旨在研究通过磁控溅射制备的纳米银涂层聚醚醚酮（PEEK）的细胞毒性和抗菌性能，为其临床应用提供理论依据。
通过磁控溅射技术在PEEK表面制备不同厚度（3、6、9和12nm）的纳米银涂层。根据纳米银涂层的厚度，将所得的涂层PEEK样品分为以下几组：PEEK-3（3nm）、PEEK-6（6nm）、PEEK-9（9nm）、PEEK-12（12nm）以及PEEK对照组。通过扫描电子显微镜（SEM）、原子力显微镜（AFM）和能量色散谱（EDS）分析观察每个样品的表面微观结构和组成。然后用接触角测量仪测量每个样品的水接触角。使用细胞计数试剂盒（CCK-8）分析涂层组（n=5）和组测试样品（n=6）、阴性对照（聚乙烯，PE）（n=6）以及阳性对照组（苯酚）（n=6）中小鼠成纤维细胞（L929）的细胞毒性。通过将变形链球菌和金黄色葡萄球菌共培养来测试样品的抗菌性能。通过SEM观察附着在样品表面的细菌。然后评估每个样品的抗菌粘附能力。
SEM和AFM分析结果表明，对照组样品表面光滑但致密。均匀的银纳米颗粒（AgNPs）和纳米银涂层均匀分布在涂层组样品的表面。与对照样品相比，随着纳米银涂层厚度的增加，纳米银涂层样品的表面粗糙度显著增加（P<0.05）。EDS分析表明，涂层样品表面不仅存在C和O，还存在Ag。此外，纳米银涂层改性后，改性样品的水接触角显著增加（P<0.01）。CCK-8细胞毒性测试结果表明，涂层样品未表现出细胞毒性。抗菌实验结果表明，纳米银涂层可以显著提高PEEK样品的抗菌活性和细菌粘附能力。
通过磁控溅射成功地在PEEK表面制备了致密且均匀的纳米银涂层。纳米银涂层的PEEK表现出增强的抗菌活性和细菌粘附能力，且无细胞毒性作用。
