Department of Physiology, Faculty of Medicine, Van Yuzuncu Yil University, Van, Turkey.
Department of Histology and Embryology, Faculty of Medicine, Van Yuzuncu Yil University, Van, Turkey.
Brain Inj. 2024 Jun 6;38(7):514-523. doi: 10.1080/02699052.2024.2324012. Epub 2024 Mar 3.
This study investigated the effects of ghrelin on oxidative stress, working memory, inflammatory parameters, and neuron degeneration.
TBI was produced with the weight-drop technique. Rats in the G+TBI and TBI+G groups received ghrelin for 7 or 2 days, respectively. The control group received saline. On the 8 day of the study, the brain and blood tissue were taken under anesthesia.
A significant increase in brain GSH-PX, MDA, IL-1β, TGF-β1, and IL-8 levels and a significant decrease in CAT levels were found in the TBI group compared to the control. Serum MDA, GSH, IL-1β, and IL-8 levels were increased with TBI. Ghrelin treatment after TBI significantly increased the serum GSH, CAT, GSH-PX, and brain GSH and CAT levels, while it significantly decreased the serum MDA, IL-1β, and brain MDA, TGF-β1, and IL-8 levels. Histological evaluations revealed that ghrelin treatment led to a reduction in inflammation, while also significantly ameliorating TBI-induced neuron damage and vascular injuries. Immunohistochemistry staining showed that GFAP staining intensity was significantly increased in the cortex and hippocampus in TBI, and GFAP immunoreactivity was decreased with ghrelin treatment.
The results from this study suggested that ghrelin may have curative effects on TBI.
本研究旨在探讨生长激素释放肽（ghrelin）对氧化应激、工作记忆、炎症参数和神经元变性的影响。
采用落体撞击法制作 TBI 模型。G+TBI 和 TBI+G 组大鼠分别接受 7 天或 2 天的 ghrelin 治疗。对照组给予生理盐水。研究第 8 天，大鼠在麻醉下取脑和血组织。
与对照组相比，TBI 组大脑 GSH-PX、MDA、IL-1β、TGF-β1 和 IL-8 水平显著升高，CAT 水平显著降低；TBI 后血清 MDA、GSH、IL-1β和 IL-8 水平升高。TBI 后给予 ghrelin 治疗可显著提高血清 GSH、CAT、GSH-PX 和大脑 GSH 和 CAT 水平，同时显著降低血清 MDA、IL-1β和大脑 MDA、TGF-β1 和 IL-8 水平。组织学评估显示，ghrelin 治疗可减轻炎症，同时显著改善 TBI 引起的神经元损伤和血管损伤。免疫组化染色显示，TBI 时皮质和海马区 GFAP 染色强度显著增加，ghrelin 治疗可降低 GFAP 免疫反应性。
本研究结果表明，ghrelin 可能对 TBI 具有治疗作用。
