Department of Laboratory, Wuhan Caidian District People's Hospital, Wuhan, China.
Brain Inj. 2024 Jun 6;38(7):524-530. doi: 10.1080/02699052.2024.2324022. Epub 2024 Mar 3.
Autophagy is recognized as a promising therapeutic target for traumatic brain injury (TBI). Crocetin is an aglycone of crocin naturally occurring in saffron and has been found to alleviate brain injury diseases. However, whether crocetin affects autophagy after TBI remains unknown. Therefore, we explore crocetin roles in autophagy after TBI.
We used a weight-dropped model to induce TBI in C57BL/6J mice. Neurological severity scoring (NSS) and grip tests were used to evaluate the neurological level of injury. Brain edema, neuronal apoptosis, neuroinflammation and autophagy were detected by measurements of brain water content, TUNEL staining, ELISA kits and western blotting.
Crocetin ameliorated neurological dysfunctions and brain edema after TBI. Crocetin reduced neuronal apoptosis and neuroinflammation and enhanced autophagy after TBI.
Crocetin alleviates TBI by inhibiting neuronal apoptosis and neuroinflammation and activating autophagy.
自噬被认为是创伤性脑损伤（TBI）有前途的治疗靶点。西红花酸是藏红花中天然存在的西红花苷的苷元，已被发现可减轻脑损伤疾病。然而，西红花酸是否影响 TBI 后的自噬尚不清楚。因此，我们探讨了西红花酸在 TBI 后自噬中的作用。
我们使用落体模型诱导 C57BL/6J 小鼠 TBI。神经严重程度评分（NSS）和握力测试用于评估损伤的神经水平。通过测量脑水含量、TUNEL 染色、ELISA 试剂盒和 Western blot 检测脑水肿、神经元凋亡、神经炎症和自噬。
西红花酸改善了 TBI 后的神经功能障碍和脑水肿。西红花酸减少了 TBI 后的神经元凋亡和神经炎症，并激活了自噬。
西红花酸通过抑制神经元凋亡和神经炎症并激活自噬来减轻 TBI。
