Adouane Emilie, Mercier Camille, Mamelle Jeanne, Willocquet Emma, Intertaglia Laurent, Burgunter-Delamare Bertille, Leblanc Catherine, Rousvoal Sylvie, Lami Raphaël, Prado Soizic
Muséum National d'Histoire Naturelle, Unité Molécules de Communication et Adaptation des Micro-Organismes MCAM, UMR 7245, CNRS, Sorbonne Université, 75005 Paris, France.
Sorbonne Université, CNRS, UAR 3579 Laboratoire de Biodiversité et Biotechnologies Microbiennes LBBM, Observatoire Océanologique, 66650 Banyuls-sur-Mer, France.
iScience. 2024 Feb 9;27(3):109176. doi: 10.1016/j.isci.2024.109176. eCollection 2024 Mar 15.
Brown macroalgae are colonized by diverse microorganisms influencing the physiology of their host. However, cell-cell interactions within the surface microbiome (epimicrobiome) are largely unexplored, despite the significance of specific chemical mediators in maintaining host-microbiome homeostasis. In this study, by combining liquid chromatography coupled to mass spectrometry (LC-MS) analysis and bioassays, we demonstrated that the widely diverse fungal epimicrobiota of the brown alga can affect quorum sensing (QS), a type of cell-cell interaction, as well as bacterial biofilm formation. We also showed the ability of the bacterial epimicrobiota to form and inhibit biofilm growth, as well as to activate or inhibit QS pathways. Overall, we demonstrate that QS and anti-QS compounds produced by the epimicrobiota are key metabolites in these brown algal epimicrobiota communities and highlight the importance of exploring this epimicrobiome for the discovery of new bioactive compounds, including potentially anti-QS molecules with antifouling properties.
褐藻被多种微生物定殖，这些微生物会影响其宿主的生理机能。然而，尽管特定化学介质在维持宿主-微生物群稳态方面具有重要意义，但表面微生物群（表生微生物群）内的细胞间相互作用在很大程度上仍未得到探索。在本研究中，通过结合液相色谱-质谱联用（LC-MS）分析和生物测定，我们证明了褐藻种类繁多的真菌表生微生物群可影响群体感应（QS），即一种细胞间相互作用，以及细菌生物膜的形成。我们还展示了细菌表生微生物群形成和抑制生物膜生长的能力，以及激活或抑制QS途径的能力。总体而言，我们证明了表生微生物群产生的QS和抗QS化合物是这些褐藻表生微生物群落中的关键代谢产物，并强调了探索这个表生微生物群以发现新的生物活性化合物的重要性，包括具有潜在防污特性的抗QS分子。
