Mamede Renato, Patinha Carla, Martins Patrícia, Ferreira da Silva Eduardo, Calado Ricardo, Ricardo Fernando
ECOMARE, CESAM - Centre for Environmental and Marine Studies, Department of Biology, University of Aveiro, Santiago University Campus, 3810-193, Aveiro, Portugal.
GEOBIOTEC, Department of Geosciences, University of Aveiro, Santiago University Campus, 3810-193, Aveiro, Portugal.
Heliyon. 2024 Feb 10;10(4):e25872. doi: 10.1016/j.heliyon.2024.e25872. eCollection 2024 Feb 29.
The fraudulent mislabelling of seafood geographic origin has been growing due to complex supply chains and growing consumer demand. To address this issue, seafood traceability tools, such as those based on elemental fingerprints (EF) of bivalve shells, have been successfully used to confirm their harvesting location. However, despite the usefulness of these methodologies, there is still room for optimization. Therefore, this study evaluated the effects of a routine procedure during bivalve shells preparation for ICP-MS analysis - their pretreatment with HO to remove organic components. More specifically, the present study evaluated the effects of HO on i) the elemental fingerprints of shells of two bivalve species ( and ) from four different locations over the north-western and the western Iberian coast, and ii) their influence on the accuracy of models (based on the EF of shells) used to confirm the geographic origin of these species. Significant differences were observed between untreated and pretreated shells of ( within location ranging from 0.0001 to 0.0011) and ( ranging from 0.0001 to 0.0007 for ) for both their elemental fingerprints as a whole and several individual elements. The accuracy of the models employed to determine the origin of the two bivalve species, using i) untreated shells, ii) pretreated shells, and iii) both pretreated and untreated shells grouped per location, was high, with the models accurately predicting the geographic origin of 100, 90 and 95% of and 95, 100 and 95% of , respectively. These results show that the shifts in the EF of bivalve shells promoted by treating them with HO prior to ICP-MS analysis did not affect the accuracy of the models used to confirm the geographic origin of both bivalve species. Therefore, the need to pre-treat bivalve shells with HO can be dismissed in future studies addressing the traceability of bivalves when using ICP-MS, thus contributing to reducing environmental impacts and economic costs associated with this procedure, as well as the time required to obtain results.
由于供应链复杂且消费者需求不断增加，海鲜地理来源的欺诈性错误标注现象日益严重。为解决这一问题，海鲜可追溯性工具，如基于双壳贝类贝壳元素指纹（EF）的工具，已成功用于确认其捕捞地点。然而，尽管这些方法很有用，但仍有优化空间。因此，本研究评估了双壳贝类贝壳用于电感耦合等离子体质谱（ICP-MS）分析的常规制备过程——用双氧水（HO）预处理以去除有机成分的效果。更具体地说，本研究评估了双氧水对以下两方面的影响：i）来自伊比利亚西北海岸和西海岸四个不同地点的两种双壳贝类（和）贝壳的元素指纹，以及ii）其对用于确认这些物种地理来源的模型（基于贝壳的元素指纹）准确性的影响。对于整体元素指纹和几个单个元素，在（同一地点范围内从0.0001至0.0011）和（对于为0.0001至0.0007）的未处理和预处理贝壳之间均观察到显著差异。用于确定两种双壳贝类物种来源的模型准确性很高，使用i）未处理贝壳、ii）预处理贝壳以及iii）按地点分组的预处理和未处理贝壳，分别准确预测了的100%、90%和95%以及的95%、100%和95%的地理来源。这些结果表明，在ICP-MS分析之前用双氧水处理双壳贝类贝壳所导致的元素指纹变化，并未影响用于确认两种双壳贝类物种地理来源的模型准确性。因此，在未来使用ICP-MS进行双壳贝类可追溯性研究时，可以不再需要用双氧水预处理双壳贝类贝壳，从而有助于减少与此过程相关的环境影响和经济成本，以及获得结果所需的时间。
